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Development of Enzyme-Linked Immunosorbent Assay for the Detection of Bt Protein in Transgenic Cotton

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Transgenic Cotton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 958))

Abstract

Enzyme-linked immunosorbent assays (ELISA) are being used extensively for the identification of Bt-protein in Bt transgenic crops. A sandwich ELISA test is the most preferable immunoassay for the quantification of Bt-protein in transgenic cotton plants. Here, we describe development of sandwich ELISA, employing polyclonal rabbit antibody as a capture antibody and HRP-labeled mouse anti-Bt protein-antibody as a detector antibody.

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Author information

Authors and Affiliations

  1. Molecular Genomics Laboratory, National Institute of Immunology, New Delhi, India

    Suchitra Kamle

  2. Structural and Computational Biology Lab, International Centre for Genetic Engineering and Biotechnology, New Delhi, India

    Abhishek Ojha

  3. Faculty of Science, Molecular Biology and Immunology Lab, School of Biotechnology, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Arvind Kumar

Authors
  1. Suchitra Kamle
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  2. Abhishek Ojha
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  3. Arvind Kumar
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Corresponding author

Correspondence to Suchitra Kamle .

Editor information

Editors and Affiliations

  1. , Department of Biology, East Carolina University, Howell Science Complex S112, Greenville, 27858, North Carolina, USA

    Baohong Zhang

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Kamle, S., Ojha, A., Kumar, A. (2013). Development of Enzyme-Linked Immunosorbent Assay for the Detection of Bt Protein in Transgenic Cotton. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 958. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-212-4_10

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  • DOI: https://doi.org/10.1007/978-1-62703-212-4_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-211-7

  • Online ISBN: 978-1-62703-212-4

  • eBook Packages: Springer Protocols

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