Abstract
The blood–nerve barrier (BNB) is one of the functional barriers sheltering the nervous system from circulating blood. It is very important to understand the cellular properties of endothelial cells of endoneurial origin because these cells constitute the bulk of the BNB. This chapter describes a standard protocol for isolating the endothelial cells forming the BNB. In addition, methods for confirming some of the barrier properties of isolated endothelial cells are also described.
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References
Poduslo JF, Curran GL, Berg CT (1994) Macromolecular permeability across the blood-nerve and blood-brain barriers. Proc Natl Acad Sci USA 91: 5705–5709
Soderfeldt B (1974) The perineurium as a diffusion barrier to protein tracers. Influence of histamine, serotonine and bradykinine. Acta Neuropathol 27: 55–60
Boddingius J (1984) Ultrastructural and histophysiological studies on the blood-nerve barrier and perineurial barrier in leprosy neuropathy. Acta Neuropathol 64: 282–296
Kanda T, Iwasaki T, Yamawaki M, Ikeda K (1997) Isolation and culture of bovine endothelial cells of endoneurial origin. J Neurosci Res 49: 769–777
Saito T, Zhang ZJ, Shibamori Y, Ohtsubo T, Noda I, Yamamoto T, Saito H (1997) P-glycoprotein expression in capillary endothelial cells of the 7th and 8th nerves of guinea pig in relation to blood-nerve barrier sites. Neurosci Lett 232: 41–44
Kanda T, Numata Y, Mizusawa H (2004) Chronic inflammatory demyelinating polyneuropathy: decreased claudin-5 and relocated ZO-1. J Neurol Neurosurg Psychiatry 75: 765–769
Sano Y, Shimizu F, Nakayama H, Abe M, Maeda T, Ohtsuki S, Terasaki T, Obinata M, Ueda M, Takahashi R, Kanda T (2007) Endothelial cells constituting blood-nerve barrier have highly specialized characteristics as barrier-forming cells. Cell Struct Funct 32: 139–147
Hosoya K, Takashima T, Tetsuka K, Nagura T, Ohtsuki S, Takanaga H, Ueda M, Yanai N, Obinata M, Terasaki T (2000) mRNA expression and transport characterization of conditionally immortalized rat brain capillary endotherial cell lines; a new in vitro BBB model for drug targeting. J Drug Target 8: 357–370
Acknowledgments
This work was supported in part by a Neuroimmunological Disease Research Committee grant from the Ministry of Health, Labour and Welfare, Japan and also by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan.
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Sano, Y., Kanda, T. (2011). Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier. In: Nag, S. (eds) The Blood-Brain and Other Neural Barriers. Methods in Molecular Biology, vol 686. Humana Press. https://doi.org/10.1007/978-1-60761-938-3_21
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DOI: https://doi.org/10.1007/978-1-60761-938-3_21
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Publisher Name: Humana Press
Print ISBN: 978-1-60761-937-6
Online ISBN: 978-1-60761-938-3
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