Abstract
Whereas the complete primary structure of a polypeptide may be established by mass spectrometry (see chapters by Wittmann-Liebold and L’Italien in this volume), this cannot be achieved by Edman degradation because this stepwise procedure does not allow discrimination between polypeptides with free or blocked COOH-terminal carboxylic groups. This shortcoming of Edman degradation must not be ignored in view of the significant biologic role of COOH-terminal modifications, especially for many regulatory pep-tides (1,2). Thus, whenever Edman degradation is used as the prinicpal method of complete amino acid sequence determination, it must be supplemented by COOH-terminal analysis.
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Spiess, J. (1986). COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_14
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DOI: https://doi.org/10.1007/978-1-59259-436-8_14
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