Novel Imaging Techniques to Analyze Panicle Architecture

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Rice Grain Quality

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1892))

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Abstract

Panicle architecture is known to directly influence grain yield in rice, and thus is an important trait for rice varietal improvement. However, spike branching consequences trigger variation in number of superior and inferior grains and thus affect grain quality. The genetics behind the length of both primary and secondary branches were studied resulting in the identification of cloned genes. Extending this knowledge to include other physiological parameters of panicle architecture is not yet well studied, and it requires high-throughput imaging techniques that are accurate. In this chapter we put the spotlight on Panicle Trait Phenoty** Tool (P-TRAP), a freely available platform independent software to analyze the panicle architecture of rice, as one of such methods that can be used to generate a comprehensive and reproducible panicle architecture data and identify superior breeding lines. P-TRAP measures 15 panicle structure and nine spikelet traits. These quantitative traits can be used in genome-wide association studies to understand their genetic basis.

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References

  1. Zeigler RS (2014) Toward food security in 2050: when I think about poverty. CSA News 59:8

    Article  Google Scholar 

  2. Vergara BS (1987) Raising the yield potential of rice. Trans Nat Acad Sci Tech 9:397–413

    Google Scholar 

  3. Khush GS (2001) Green revolution: the way forward. Nat Rev Genet 2:815–822

    Article  CAS  Google Scholar 

  4. Khush GS (1995) Breaking the yield frontier of rice. Geo J 35:329–332

    Google Scholar 

  5. Crowell S, Falcão AX, Shah A, Wilson Z, Greenberg AJ, McCouch SR (2014) High-resolution inflorescence phenoty** using a novel image-analysis pipeline, PANorama. Plant Physio 166:479–495

    Article  Google Scholar 

  6. FM AL-T, Adam H, Anjos A, Lorieux M, Larmande P, Ghesquière A, Jouannic S, Shahbazkia HR (2013) P-TRAP: a panicle trait phenoty** tool. BMC Plant Biol 13:122

    Article  Google Scholar 

  7. **ng Y, Zhang Q (2010) Genetic and molecular bases of rice yield. An Rev Plant Biol 61:421–442

    Article  CAS  Google Scholar 

  8. Zhu Z, Tan L, Fu Y, Liu F, Cai H, **e D, Wu F, Jianzhong F, Wu J, Matsumoto T, Sun C (2013) Genetic control of inflorescence architecture during rice domestication. Nat Commun 4:2200

    Article  Google Scholar 

  9. Mohapatra PK, Panigrahi R, Turner NC (2011) Physiology of spikelet development on the rice panicle: is manipulation of apical dominance crucial for grain yield improvement? Adv Agro 10:333–359

    Article  Google Scholar 

  10. Zhang X, Alim N, Lin Z, Liu Z, Li G, Wang Q, Wang S, Ding Y (2013) Analysis of variations in white-belly and white-core rice kernels within a panicle and the effect of panicle type. J Integ Agr Adv Online Pub:1–11

    Google Scholar 

  11. Matsue Y, Odahara K, Hiramatsu M (1995) Differences in amylose content, amylographic characteristics and storage proteins of grains on primary and secondary rachis-branches in rice. Japan J Crop Sci 64(3):601–1606

    Article  CAS  Google Scholar 

  12. Umemoto T, Nakamura Y, Ishikura N (1994) Effect of grain location on the panicle on activities involved in starch synthesis in rice endosperm. Phytochemistry 36:843–847

    Article  CAS  Google Scholar 

  13. Iwasaki Y, Mae T, Makino A, Ohira K, Ojima K (1992) Nitrogen accumulation in the inferior spikelet of rice ear during ripening. Soil Sci Plant Nutri 38:517–525

    Article  CAS  Google Scholar 

  14. Liu ZH, Cheng FM, Cheng WD, Zhang GP (2005) Positional variations in phytic acid and protein content within a panicle of japonica rice. J Cereal Sci 41:297–303

    Article  CAS  Google Scholar 

  15. Ohsumi A, Takai T, Ida M, Yamamoto T, Arai-Sanoh YM, Ando T, Kondo M (2011) Evaluation of yield performance in rice near-isogenic lines with increased spikelet number. Field Crops Res 120:68–75

    Article  Google Scholar 

  16. Peng S, Cassman KG, Virmani SS, Sheehy J, Khush GS (1999) Yield potential trends of tropical rice since the release of IR8 and the challenge of increasing rice yield potential. Crop Sci 39:1552–1559

    Article  Google Scholar 

  17. Duan L, Yang W, Huang C, Liu Q (2011) A novel machine-vision-based facility for the automatic evaluation of yield-related traits in rice. Plant Methods 7:44

    Article  Google Scholar 

  18. Huang C, Yang W, Duan L, Jiang N, Chen G, **ong L, Qian L (2013) Rice panicle length measuring system based on dual-camera imaging. Comp Elect Agri 98:158–165

    Article  Google Scholar 

  19. Tanabata T, Shibaya T, Hori K, Ebana K, Yano M (2012) SmartGrain: high throughput phenoty** software for measuring seed shape through image analysis. Plant Physiol 160:1871–1880

    Article  CAS  Google Scholar 

  20. Ikeda M, Hirose Y, Shibata Y, Yamamura T, Komura T, Doi K, Ahikari M, Matsuoka M, Kitano H (2010) Analysis of rice panicle traits and detection of QTLs using an image analyzing method. Breed Sci 60:55–64

    Article  Google Scholar 

  21. Zhao S, Gu J, Zhao Y, Hassan M, Li Y, Ding W (2013) A method for estimating spikelet number per panicle: integrating image analysis and a 5-point calibration model. Sci Rep 5:16241

    Article  Google Scholar 

  22. Duan L, Huang C, Chen G, **ong L, Liu Q, Yang W (2015) Determination of rice panicle numbers during heading by multi-angle imaging. The Crop J 3:211–219

    Article  Google Scholar 

  23. Jhala T (2015) X-ray computed tomography to study rice (Oryza sativa L.) panicle development. J Expt’l Bot 66(21):6819–6825

    Article  CAS  Google Scholar 

  24. **ong X, Duan L, Liu L, Tu H, Yang P, Wu D, Chen G, **ong L, Yang W, Liu Q (2017) Panicle-SEG: a robust image segmentation method for rice panicles in the field based on deep learning and superpixel optimization. Plant Methods 13:104

    Article  Google Scholar 

  25. Rebolledo MC, Peña AL, Duitama J, Cruz DF, Dingkuhn M, Grenier C, Tohme J (2016) Combining image analysis, genome wide association studies and different field trials to reveal stable genetic regions related to panicle architecture and the number of spikelets per panicle in rice. Front Plant Sci 7:1384

    Article  Google Scholar 

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Acknowledgment

This work has been supported under the CGIAR thematic area Global Rice Agri-Food System CRP, RICE, Stress-Tolerant Rice for Africa and South Asia (STRASA) Phase III, and Australian Centre for International Agricultural Research (Project ID CIM/2016/046) funding.

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Correspondence to Roslen Anacleto .

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Pasion, E., Aguila, R., Sreenivasulu, N., Anacleto, R. (2019). Novel Imaging Techniques to Analyze Panicle Architecture. In: Sreenivasulu, N. (eds) Rice Grain Quality. Methods in Molecular Biology, vol 1892. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8914-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8914-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8912-6

  • Online ISBN: 978-1-4939-8914-0

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