Abstract
Glutamate uptake has a predominant role in the regulation of glutamate homeostasis mainly for the prevention of hyperexcitability and excitotoxicity which induce neuronal dysfunctions and neurological diseases. An evaluation of in vivo glutamate transport can enable to verify the functional impact of alterations previously found in in vitro and ex vivo preparations. The use of brain microdialysis to sample the extracellular medium has allowed to quantify the dynamic uptake of glutamate through the active transporters present on cell membranes which maintain glutamate homeostasis. We describe two quantitative methods based on the application of labeled or unlabeled glutamate through a microdialysis probe to measure the in vivo uptake of glutamate in a discrete brain area of freely moving rats, accompanied with step by step details. Advantages and drawbacks of the methods are also discussed on both methodological and physiological bases, by relying on previous studies carried out in experimental pathological or pharmacological models.
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The authors thank the Institut National de la Santé et de la Recherche Médicale (INSERM), the Centre National de la Recherche Scientifique (CNRS), and the Université Claude Bernard Lyon 1.
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Parrot, S., Touret, M., Denoroy, L. (2018). In Vivo Determination of Glutamate Uptake by Brain Microdialysis. In: Parrot, S., Denoroy, L. (eds) Biochemical Approaches for Glutamatergic Neurotransmission. Neuromethods, vol 130. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7228-9_14
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