Abstract
Unilateral common carotid artery occlusion (CCAO) is a standardized method to initiate collateral artery growth (arteriogenesis) in mouse brain. After CCAO is induced, blood circulation in the circle of Willis is changed and increases shear stress, which triggers increased arterial diameter and improvements in cerebrovascular reserve capacity. Functional improvement can be quantified after experimentally induced stroke by external middle cerebral artery occlusion (MCAO). Stroke volume is evaluated by standard tetrazolium chloride (TTC) staining. Here, we describe in vivo methods of CCAO and MCAO in detail and also the evaluation of stroke volume by TTC staining.
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Acknowledgements
We thank Prof. James Faber and his colleagues (The University of North Carolina at Chapel Hill School of Medicine) for professionally teaching us the methodological details of the external MCAO procedure.
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Duelsner, A., Gatzke, N., Persson, A.B., Buschmann, I.R. (2014). Induction of Cerebral Arteriogenesis in Mice. In: Milner, R. (eds) Cerebral Angiogenesis. Methods in Molecular Biology, vol 1135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0320-7_11
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DOI: https://doi.org/10.1007/978-1-4939-0320-7_11
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0319-1
Online ISBN: 978-1-4939-0320-7
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