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Identification and Characterization of Antigen-Specific T-Cells in Viral Infections

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Intracellular Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2813))

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Abstract

Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantification and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.

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Acknowledgments

We thank Sara Suárez Hernández for making the murine rotavirus-specific tetramers.

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Author notes

  1. Darya Trubach and Konjit Getachew Muleta contributed equally with all other contributors.

Authors and Affiliations

  1. Section for Experimental and Translational Immunology, Institute for Health Technology, Technical University of Denmark (DTU), Kongens Lyngby, Denmark

    Darya Trubach, Katharina Lahl & Sunil Kumar Saini

  2. Immunology Section, Lund University, Lund, Sweden

    Konjit Getachew Muleta & Katharina Lahl

Authors
  1. Darya Trubach
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  2. Konjit Getachew Muleta
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  3. Katharina Lahl
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  4. Sunil Kumar Saini
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Corresponding author

Correspondence to Sunil Kumar Saini .

Editor information

Editors and Affiliations

  1. Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada

    Aneesh Thakur

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Trubach, D., Muleta, K.G., Lahl, K., Saini, S.K. (2024). Identification and Characterization of Antigen-Specific T-Cells in Viral Infections. In: Thakur, A. (eds) Intracellular Pathogens. Methods in Molecular Biology, vol 2813. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3890-3_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3890-3_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3889-7

  • Online ISBN: 978-1-0716-3890-3

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