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Some Guiding Principles for a “Simple” Correlative Light Electron Microscopy Experiment

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Imaging Cell Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2800))

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Abstract

In recent years, Correlative Multimodal Imaging (CMI) has become an “en vogue” technique and a bit of a buzzword. It entails combining information from different imaging modalities to extract more information from a sample that would otherwise not be possible from each individual technique. The best established CMI technology is correlative light and electron microscopy (CLEM), which applies light and electron microscopy on the exact same sample/structure. In general, it entails the detection of fluorescently tagged proteins or structures by light microscopy and subsequently their relative intracellular localization is determined with nanometer resolution using transmission electron microscopy (TEM). Here, we describe the different steps involved in a “simple” CLEM approach. We describe the overall workflow, instrumentation, and basic principles of sample preparation for a CLEM experiment exploiting stable expression of fluorescent proteins.

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Acknowledgments

We would like to thank the Wolfson Bioimaging Facility at the University of Bristol for their expert technical support. This work was funded by the Academy of Finland under the award number 332615 to Elina Mäntylä.

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Authors and Affiliations

  1. BioMediTech, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland

    Elina Mäntylä

  2. School of Biochemistry, University of Bristol, Bristol, UK

    Paul Verkade

Authors
  1. Elina Mäntylä
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  2. Paul Verkade
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Corresponding author

Correspondence to Paul Verkade .

Editor information

Editors and Affiliations

  1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Christoph Wuelfing

  2. Computational Biology Department, Carnegie Mellon University, Pittsburgh, PA, USA

    Robert F. Murphy

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mäntylä, E., Verkade, P. (2024). Some Guiding Principles for a “Simple” Correlative Light Electron Microscopy Experiment. In: Wuelfing, C., Murphy, R.F. (eds) Imaging Cell Signaling. Methods in Molecular Biology, vol 2800. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3834-7_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3834-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3833-0

  • Online ISBN: 978-1-0716-3834-7

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