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Protocols for Building and Producing High Diversity Peptide Phage Display Libraries

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Phage Engineering and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2793))

Abstract

Phage display is an important technology to study protein-protein interaction and protein evolution, with applications in basic science and applied biotechnology, such as drug discovery and the development of targeted therapies. However, in order to be successful during a phage display screening, it is paramount to have good phage libraries. Here, we described detailed procedures to generate peptide phage display libraries with high diversity and billions of transformants.

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Acknowledgement

This work was supported by Sao Paulo State Agency (FAPESP) grant 2019/25.828-8 to RJG.

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Authors and Affiliations

  1. Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil

    Ricardo Jose Giordano & Lilian Costa Alecrim

Authors
  1. Ricardo Jose Giordano
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  2. Lilian Costa Alecrim
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Corresponding author

Correspondence to Ricardo Jose Giordano .

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Editors and Affiliations

  1. Cellular Signaling Laboratory International Research, Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, China

    Huan Peng

  2. College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, China

    Jianfeng Liu

  3. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA

    Irene A. Chen

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Giordano, R.J., Alecrim, L.C. (2024). Protocols for Building and Producing High Diversity Peptide Phage Display Libraries. In: Peng, H., Liu, J., Chen, I.A. (eds) Phage Engineering and Analysis. Methods in Molecular Biology, vol 2793. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3798-2_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3798-2_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3797-5

  • Online ISBN: 978-1-0716-3798-2

  • eBook Packages: Springer Protocols

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