Abstract
The β-barrel assembly machinery (BAM) complex in Gram-negative bacteria facilitates the assembly of β-barrel proteins into the outer membrane. Understanding the protein-protein interactions within this complex is essential for unravelling its functional mechanisms. Here, we present the use of neutron reflectometry for investigating the organization of β-barrel membrane protein complexes in the membrane environment. The spatial organization, protein positioning, protein-lipid interactions, and conformational changes within the complex can be elucidated by this method.
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Acknowledgements
The work was supported by grants from Australian NHMRC. H.H.S. is an Australian NHMRC Career Development Research Fellow (GNT1106798).
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Lai, X., Shen, HH. (2024). Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry. In: Ieva, R. (eds) Transmembrane β-Barrel Proteins. Methods in Molecular Biology, vol 2778. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3734-0_17
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DOI: https://doi.org/10.1007/978-1-0716-3734-0_17
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