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A Mammalian-Based Synthetic Biology Toolbox to Engineer Membrane–Membrane Interfaces

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Mammalian Synthetic Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2774))

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Abstract

Intercellular membrane–membrane interfaces are compartments with specialized functions and unique biophysical properties that are essential in numerous cellular processes including cell signaling, development, and immunity. Using synthetic biology to engineer or to create novel cellular functions in the intercellular regions has led to an increasing need for a platform that allows generation of functionalized intercellular membrane–membrane interfaces. Here, we present a synthetic biology platform to engineer functional membrane–membrane interfaces using a pair of dimerizing proteins in both cell-free and cellular environments. We envisage this platform to be a helpful tool for synthetic biologists who wish to engineer novel intercellular signaling and communication systems.

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Author information

Authors and Affiliations

  1. Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA

    Hossein Moghimianavval & Allen P. Liu

  2. Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA

    Sonisilpa Mohapatra

  3. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA

    Allen P. Liu

  4. Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA

    Allen P. Liu

  5. Department of Biophysics, University of Michigan, Ann Arbor, MI, USA

    Allen P. Liu

Authors
  1. Hossein Moghimianavval
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  2. Sonisilpa Mohapatra
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  3. Allen P. Liu
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Corresponding author

Correspondence to Allen P. Liu .

Editor information

Editors and Affiliations

  1. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Francesca Ceroni

  2. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Karen Polizzi

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Moghimianavval, H., Mohapatra, S., Liu, A.P. (2024). A Mammalian-Based Synthetic Biology Toolbox to Engineer Membrane–Membrane Interfaces. In: Ceroni, F., Polizzi, K. (eds) Mammalian Synthetic Systems. Methods in Molecular Biology, vol 2774. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3718-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3718-0_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3717-3

  • Online ISBN: 978-1-0716-3718-0

  • eBook Packages: Springer Protocols

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