Abstract
Microfluidic flow focusing provides an efficient approach to the generation of nanoscale lipid vesicles of tunable size and low size variance. Scalable nanoliposome synthesis over a wide range of production rates can be readily achieved using a high aspect ratio flow focusing device fabricated by widely available additive manufacturing methods. Here we detail methods for the manufacture and operation of a 3D-printed microfluidic flow focusing technology enabling the synthesis of liposomes with modal diameters ranging from ca. 50–200 nm at production rates up to several hundred milligrams lipid per hour.
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Acknowledgments
This work was supported by the US National Science Foundation through NSF grant CMMI1562468.
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513594_3_En_7_MOESM1_ESM.stl
SI1_device_design_file.stl – Microfluidic flow focusing device CAD design file in standard stereolithography (STL) format (STL 1794 kb)
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Han, J.Y., Chen, Z., Devoe, D.L. (2023). Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing. In: D'Souza, G.G., Zhang, H. (eds) Liposomes. Methods in Molecular Biology, vol 2622. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2954-3_7
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DOI: https://doi.org/10.1007/978-1-0716-2954-3_7
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