Abstract
N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Fezza, F., Mastrangelo, N., Maccarrone, M. (2023). Radiometric Assay of NAPE-PLD Activity. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_18
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DOI: https://doi.org/10.1007/978-1-0716-2728-0_18
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2727-3
Online ISBN: 978-1-0716-2728-0
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