Abstract
Co-immunoprecipitation is a well-established technique for determining whether two proteins interact. It is based on the principle that by pulling down one protein, you will also obtain any other proteins that exist in a complex with that protein. It is a relatively simple technique that does not require expensive reagents or materials. It is however, not without its limitations and some of these will be discussed here along with a step-by-step guide to performing and analyzing co-immunoprecipitation experiments.
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Evans, I.M., Paliashvili, K. (2022). Co-immunoprecipitation Assays. In: Fiedler, L.R., Pellet-Many, C. (eds) VEGF Signaling. Methods in Molecular Biology, vol 2475. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2217-9_8
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DOI: https://doi.org/10.1007/978-1-0716-2217-9_8
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