Abstract
Preclinical mouse models of lung cancer have been vital experimental tools to elucidate cancer biology and test novel therapeutic regimens. Two main models are most commonly used—genetically engineered mouse models and xenograft transplantation models. The most common xenograft model employs subcutaneous transplantation of tumor cells. However, the subcutaneous space is a foreign environment to lung cancer cells and does not appropriately model the tumor-stromal interactions of endogenous lung cancers. Here, we present an orthotopic mouse model of lung cancer that utilizes direct injection of cancer cells into the lung parenchyma that allows many potential studies including interactions of lung fibroblast Hedgehog pathway activity and tumor epithelia. The protocol describes this procedure and its potential applications for lung cancer research.
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Acknowledgments
The work is supported in part by University of Texas Southwestern, National Cancer Institute (1R01CA196851), the Department of Defense (W81XWH-14-1-0338), American Cancer Society (ACS-IRG-02-196), and the Sidney Kimmel Foundation for Cancer Research (SKF-14-057) to JK. Alexandra N. Wilson and Baozhi Chen contributed equally to this work.
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Wilson, A.N., Chen, B., Liu, X., Kurie, J.M., Kim, J. (2022). A Method for Orthotopic Transplantation of Lung Cancer in Mice. In: Li, X. (eds) Hedgehog Signaling. Methods in Molecular Biology, vol 2374. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1701-4_20
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DOI: https://doi.org/10.1007/978-1-0716-1701-4_20
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