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DNA Damage in Liver Cells of the Tilapia Fish Oreochromis mossambicus Larva Induced by the Insecticide Cyantraniliprole at Sublethal Doses During Chronic Exposure

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Environmental Toxicology and Toxicogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2326))

Abstract

Cyantraniliprole can effectively control lepidopteran pests and has been used all over the world. In general, the risk of cyantraniliprole seems low for fish, but the toxicity selectivity among different fish species was not clear. Here, we present the methods for the acute toxicity and chronic effects of cyantraniliprole by using juvenile tilapia (Oreochromis mossambicus). Based on this test, 96 h LC50 of cyantraniliprole to tilapia was 38.0 mg/L. After exposed for 28 days, specific growth rates of the blank control, solution control, and the treatments of 0.037, 0.37 and 3.7 mg/L of cyantraniliprole were 1.14, 0.95, 0.93, 0.82, and 0.70% per day, respectively. The results of micronucleus experiment and single cell gel electrophoresis showed that cyantraniliprole damaged DNA in liver cells of tilapia larvae. Quantitative PCR results showed that cyantraniliprole could induce the upregulation of Rpa 3 that is responsible for the DNA repair. The significant downregulation of Chk 2 gene was related to p53 pathway. It is therefore proposed that cyantraniliprole causes DNA damage in liver cells of tilapia and activates DNA damage and repair pathways.

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Authors and Affiliations

  1. Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou, China

    Yongmei Fan, Chengbin Xu & Weiguo Miao

Authors
  1. Yongmei Fan
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  2. Chengbin Xu
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  3. Weiguo Miao
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Corresponding author

Correspondence to Weiguo Miao .

Editor information

Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    ** Pan

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Fan, Y., Xu, C., Miao, W. (2021). DNA Damage in Liver Cells of the Tilapia Fish Oreochromis mossambicus Larva Induced by the Insecticide Cyantraniliprole at Sublethal Doses During Chronic Exposure. In: Pan, X., Zhang, B. (eds) Environmental Toxicology and Toxicogenomics. Methods in Molecular Biology, vol 2326. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1514-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1514-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1513-3

  • Online ISBN: 978-1-0716-1514-0

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