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High-Throughput DNA Isolation in Vegetable Crops for Genomics Applications

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Crop Breeding

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2264))

Abstract

Isolating high-quality DNA is essential for several applications in molecular biology and genomics. Performing whole-genome sequencing in crops and development of reduced representation genomic libraries for genoty** require precise standard on DNA in terms of concentration and purity. For screening large populations it is essential to increase the extraction throughput at affordable costs. In this chapter a homemade protocol is provided that is able to isolate in 96-well plates 198 samples of DNA in a single extraction. The method has been validated in tomato and pepper and can be applied in several vegetable species.

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Reference

  1. Fulton TM, Chunwongse J, Tanksley SD (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13:207–209

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Author information

Authors and Affiliations

  1. Council for Agricultural Research and Economics - Research Centre for Vegetable and Ornamental Crops (CREA-OF), Pontecagnano, SA, Italy

    Pasquale Tripodi

  2. CREA Research Centre for Vegetable and Ornamental Crops, Pontecagnano Faiano, Italy

    Giovanna Festa

Authors
  1. Pasquale Tripodi
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  2. Giovanna Festa
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Corresponding author

Correspondence to Pasquale Tripodi .

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Editors and Affiliations

  1. Council for Agricultural Research and Economics - Research Centre for Vegetable and Ornamental Crops (CREA-OF), Pontecagnano, Salerno, Italy

    Pasquale Tripodi

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Tripodi, P., Festa, G. (2021). High-Throughput DNA Isolation in Vegetable Crops for Genomics Applications. In: Tripodi, P. (eds) Crop Breeding. Methods in Molecular Biology, vol 2264. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1201-9_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1201-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1200-2

  • Online ISBN: 978-1-0716-1201-9

  • eBook Packages: Springer Protocols

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