Abstract
AJLec (18.5 kDa), a novel galactose-specific lectin, was isolated from the sea anemone Anthopleura japonica. AJLec demonstrates specificity for galactose monomers and β-linked terminal galactose residues in complex carbohydrates but not for N-acetylgalactosamine (GalNAc) which is commonly recognized by the galactose-binding lectins. Here, we have described the characteristics and process of extraction and purification of AJLec from Anthopleura japonica, which is useful for the analysis of complex carbohydrates.
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References
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Unno, H. (2020). Purification of AJLec: A Novel Galactose-Specific Lectin from the Sea Anemone Anthopleura japonica. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_56
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_56
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0430-4
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