Abstract
Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.
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Acknowledgement
We gratefully acknowledge financial support from the Funds for Creative Research Groups of China (81621003) and National Key Basic Research Program of China (2013CB933903).
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Zhu, W., Xu, Y., **, R., Wu, C., Ai, H. (2020). MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles. In: Basel, M., Bossmann, S. (eds) Cell Tracking. Methods in Molecular Biology, vol 2126. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0364-2_10
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DOI: https://doi.org/10.1007/978-1-0716-0364-2_10
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