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Live Imaging of Arabidopsis Axillary Meristems

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Plant Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2094))

Abstract

Axillary meristems (AMs) are established postembryonically at the leaf axils and can develop into lateral branches. The initiation of AMs establishes new growth axis and is of primary importance for understanding plant development. Understanding plant development requires live imaging of morphogenesis and gene expression. However, AMs are embedded in the leaf axil, making it challenging to perform live imaging. In this chapter, we describe how to prepare and culture Arabidopsis thaliana leaves in vitro, to perform one-time or time-lapse imaging of AM initiation with a confocal microscope.

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Acknowledgments

We thank Max Bush for schematics and Muhammad Sajjad for proofreading. Research in the authors’ laboratory is supported by the National Natural Science Foundation of China grants 31430010, 31861130355, and 31825002, the Key Project of the Frontier Science of the Chinese Academy of Sciences grant ZDBS-LY-SM012, and a Royal Society Newton Advanced Fellowship (NAF/R1/180125).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Bei**g), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Bei**g, China

    Bihai Shi, Hongli Wang & Yuling Jiao

  2. University of Chinese Academy of Sciences, Bei**g, China

    Bihai Shi, Hongli Wang & Yuling Jiao

Authors
  1. Bihai Shi
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  2. Hongli Wang
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  3. Yuling Jiao
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Corresponding author

Correspondence to Yuling Jiao .

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Editors and Affiliations

  1. Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, Abu Dhabi, United Arab Emirates

    Muhammad Naseem

  2. Department of Bioinformatics, University of Würzburg, Würzburg, Germany

    Thomas Dandekar

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Shi, B., Wang, H., Jiao, Y. (2020). Live Imaging of Arabidopsis Axillary Meristems. In: Naseem, M., Dandekar, T. (eds) Plant Stem Cells. Methods in Molecular Biology, vol 2094. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0183-9_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0183-9_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0182-2

  • Online ISBN: 978-1-0716-0183-9

  • eBook Packages: Springer Protocols

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