SpringerLink
Log in

Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands

  • Protocol
  • First Online:
Stem Cells and Lineage Commitment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2736))

Abstract

Salivary gland myoepithelial cells regulate salivary secretion and have been implicated in the histological diversity of salivary gland tumors. However, isolation of myoepithelial cells has been difficult owing to a lack of detailed functional analysis and cell surface markers. Therefore, we aimed to isolate myoepithelial cells from adult mouse submandibular glands using the epithelial marker EpCAM and cell adhesion factor CD49f as indicators and characterize them via sphere-forming culture. Functional analysis of specific gene expression in myoepithelial cells is possible via cell transfection experiments using the piggyBac transposon vector system. Here, we describe detailed methods and tips for the isolation and functional analysis of myoepithelial cells.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
EUR 44.95
Price includes VAT (Germany)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
EUR 128.39
Price includes VAT (Germany)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
EUR 171.19
Price includes VAT (Germany)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Makarenkova HP, Dartt DA (2015) Myoepithelial cells: their origin and function in lacrimal gland morphogenesis, homeostasis, and repair. Curr Mol Biol Reports 1:115–123. https://doi.org/10.1007/s40610-015-0020-4

    Article  Google Scholar 

  2. Emmerson E, Knox SM (2018) Salivary gland stem cells: a review of development, regeneration and cancer. Genesis 56:e23211. https://doi.org/10.1002/dvg.23211

    Article  PubMed  PubMed Central  Google Scholar 

  3. Shackleton M et al (2006) Generation of a functional mammary gland from a single stem cell. Nature 439:84–88. https://doi.org/10.1038/nature04372

    Article  CAS  PubMed  Google Scholar 

  4. Sale S, Lafkas D, Artavanis-Tsakonas S (2013) Notch2 genetic fate map** reveals two previously unrecognized mammary epithelial lineages. Nat Cell Biol 15:451–460. https://doi.org/10.1038/ncb2725

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Prater D et al (2014) Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942–950

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Centonze A et al (2020) Heterotypic cell-cell communication regulates glandular stem cell multipotency. Nature 584:608–613. https://doi.org/10.1038/s41586-020-2632-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Mattingly A, Finley JK, Knox SM (2015) Salivary gland development and disease. Wiley Interdiscip Rev Dev Biol 4:573–590. https://doi.org/10.1002/wdev.194

    Article  PubMed  PubMed Central  Google Scholar 

  8. Yasuhara R et al (2022) Role of Snai2 and notch signaling in salivary gland myoepithelial cell fate. Lab Investig 102:1245–1256. https://doi.org/10.1038/s41374-022-00814-7

    Article  CAS  PubMed  Google Scholar 

  9. Yoon YJ et al (2022) Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands. Nat Commun 13:3291. https://doi.org/10.1038/s41467-022-30934-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Donehower LA et al (1992) Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature 356:215–221. https://doi.org/10.1038/356215a0

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo, Japan

    Rika Yasuhara, Seya Kang, Rino Tokumasu & Kenji Mishima

Authors
  1. Rika Yasuhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seya Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rino Tokumasu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kenji Mishima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Rika Yasuhara or Kenji Mishima .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2023 Springer Science+Business Media, LLC

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Yasuhara, R., Kang, S., Tokumasu, R., Mishima, K. (2023). Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands. In: Turksen, K. (eds) Stem Cells and Lineage Commitment. Methods in Molecular Biology, vol 2736. Humana, New York, NY. https://doi.org/10.1007/7651_2022_472

Download citation

  • DOI: https://doi.org/10.1007/7651_2022_472

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3536-0

  • Online ISBN: 978-1-0716-3537-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation