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Antimicrobial effects of sophorolipid in combination with lactic acid against poultry-relevant isolates

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Abstract

The objective of this study was to evaluate the antibacterial effect of sophorolipid in combination with lactic acid against relevant bacteria isolated from the poultry industry. Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica, and Escherichia coli were isolated from chicken meat and antibacterial tests with sophorolipid and lactic acid were performed. Checkerboard, time-kill, and scanning electron microscopy analyses were used to confirm the antibacterial action and the combined effects. Although no inhibitory effects were observed for E. coli and Salmonella, these compounds presented antibacterial activity against L. monocytogenes and S. aureus. Additionally, sophorolipid and lactic acid were not cytotoxic at the concentrations used in the tests. The combination of sophorolipid and lactic acid resulted in an additive interaction, reducing the concentration of the active compounds needed for effectiveness against S. aureus and L. monocytogenes, to 50% and 75%, respectively. These findings lead to the possibility of develo** a new, sustainable, and natural antimicrobial solution that is considered noncytotoxic and has wide applicability in the poultry industry to reduce substantial losses in this sector.

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All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by the National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES).

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, State University of Londrina, Mailbox 10.011, Londrina, Paraná, 86057-970, Brazil

    Victória Akemi Itakura Silveira & Maria Antonia Pedrine Colabone Celligoi

  2. Department of Microbiology, State University of Londrina, Mailbox 10.011, Londrina, Paraná, 86057-970, Brazil

    Renata Katsuko Takayama Kobayashi, Admilton Gonçalves de Oliveira Junior & Gerson Nakazato

  3. Department of General Biology, State University of Londrina, Mailbox 10.011, Londrina, Paraná, 86057-970, Brazil

    Mario Sérgio Mantovani

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  1. Victória Akemi Itakura Silveira
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Contributions

Victória Akemi Itakura Silveira: conceptualization, formal analysis, investigation, methodology, visualization, writing—original draft preparation, writing—review & editing. Renata Katsuko Takayama Kobayashi: conceptualization, methodology, resources, writing—review & editing. Admilton Gonçalves de Oliveira Junior: conceptualization, methodology, resources, writing—review & editing. Mario Sérgio Mantovani: conceptualization, methodology, resources, writing—review & editing. Gerson Nakazato: conceptualization, methodology, resources, writing—review & editing. Maria Antonia Pedrine Colabone Celligoi: conceptualization, funding acquisition, methodology, project administration, resources, supervision, writing—review & editing.

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Correspondence to Maria Antonia Pedrine Colabone Celligoi.

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This study was approved by the Human Ethics Committee of State University of Londrina (CAAE 47661115.0.0000.5231, No. 1.268.019).

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Silveira, V.A.I., Kobayashi, R.K.T., de Oliveira Junior, A.G. et al. Antimicrobial effects of sophorolipid in combination with lactic acid against poultry-relevant isolates. Braz J Microbiol 52, 1769–1778 (2021). https://doi.org/10.1007/s42770-021-00545-9

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