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Auxin positively regulates nitrogen remobilization in cucumber leaves

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Abstract

Nitrogen (N) remobilization is an important N metabolic process in plants. The objective of this study was to increase our understanding of the relationship between low N tolerance and N remobilization in cucumber. Two cucumber cultivars, namely D0328 and D0422, were included in the analyses, the former of which displays a stronger capacity for low N tolerance. Source leaf transcriptomes were analyzed, which revealed differentially expressed genes in a number of interesting pathways, including amino acid biosynthesis, tyrosine and tryptophan biosynthesis, plant hormone signal transduction, and E3 ligase activity, implying that these pathways are involved in low N tolerance in cucumber. In agreement with transcriptome data, old D0328 leaves had significantly higher tryptophan and indole-3-acetic acid contents than old D0422 leaves under low N conditions. N remobilization efficiency in old leaves was increased by naphthaleneacetic acid treatment, whereas it was repressed by antiauxins α-(p-chlorophenoxy) isobutyric acid treatment. Taken together, the results of this study demonstrate that auxin promotes N remobilization in cucumber leaves during the reproductive stage of plant development and this positive regulation is associated with low N tolerance in cucumber.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 31101545); Program for New Century Excellent Talents in Heilongjiang Provincial University (No. 1452G03); University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. UNPYSCT-2015001); ‘Academic backbone’ Project of Northeast Agricultural University (No. 16XG05); ‘Young Talents’ Project of Northeast Agricultural University (No. 14QC07); Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture/Northeast Agricultural University (No. neauhc201601).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Yalin Du, Lianxue Fan, Chunyu Tian & Tao Wu

  2. Horticulture College, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Yalin Du, Lianxue Fan, Chunyu Tian & Tao Wu

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  1. Yalin Du
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  2. Lianxue Fan
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  3. Chunyu Tian
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  4. Tao Wu
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Correspondence to Tao Wu.

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Du, Y., Fan, L., Tian, C. et al. Auxin positively regulates nitrogen remobilization in cucumber leaves. Hortic. Environ. Biotechnol. 59, 189–198 (2018). https://doi.org/10.1007/s13580-018-0020-4

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