Abstract
We investigated the effects of various ratios of red to far-red light-emitting diodes (LEDs) on growth characteristics, physiological response, and cell division of red leaf lettuce. Sixteen-day-old lettuce seedlings were transferred into growth chambers and cultivated under various ratios of red (R) and far-red (FR) LEDs (R/FR = 0.7, 1.2, 4.1, and 8.6), only red LEDs (RED), or fluorescent lamps (control) for 22 days. Growth characteristics were measured at 11 and 22 days of treatment. In addition, cell division rate, epidermal cell density, chlorophyll fluorescence, and photosynthesis of leaves were analyzed. Fresh and dry weights and leaf area in all R/FR treatments were higher than those in the control at 22 days of treatment. The R/FR 1.2 had the highest values among R/FR treatments. The number of leaves appeared to increase as R/FR ratio increased. The specific leaf weights in the R/FR ratio of 0.7, 1.2, and 8.6 were similar to the control at 22 days of treatment. The SPAD values in all R/FR treatments were lower than that in the control. All R/FR treatments led to a longer leaf shape than the control. The percentage of cells in the G2M phase, indicating the cell division rate, increased in the R/FR treatments after 4 days of treatment, which supported the growth improvement in the R/FR treatments. The Fv/Fm and the photosynthetic rate in all treatments decreased due to the absence of blue light. The results of this study suggest that the supplementation with far-red LEDs should be considered when designing artificial lighting systems for closed-type plant factories since far-red light affects the vegetative growth of leafy vegetables such as lettuce.
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Literature Cited
Alothman, M., R. Bhat, and A.A. Karim. 2009. Effects of radiation processing on phytochemicals and antioxidants in plants produce. Trends Food Sci. Technol. 20:201–212.
Carvalho, R.F., M. Takaki, and R.A. Azevedo. 2011. Plant pigments: The many faces of light perception. Acta Physiol. Plant. 33:241–248.
Ceulemans, R., L. van Praet, and X.N. Jiang. 1995. Effects CO2 enrichment, leaf position and clone on stomatal index and epidermal cell density in poplar (Populus). New Phytol. 131:99–107.
Craig, D.S. and E.S. Runkle. 2013. A moderate to high red to far-red light ratio from light-emitting diodes controls flowering of short-day plants. J. Am. Soc. Hortic. Sci. 138:167–172.
Cummings, I.G., J.B. Reid, and A. Koutoulis. 2007. Red to far-red ratio correction in plant growth chambers-growth responses and influence of thermal load on garden pea. Physiol. Plant. 131:171–179.
Franklin, K.A. and G.C. Whitelam. 2005. Phytochromes and shadeavoidance responses in plants. Ann. Bot. 96:169–175.
Jeong, S.W., J.C. Park, and M.R. Huh. 2009. Growth inhibition, seedling damage, and antioxidant change of cucumber plug-transplants in response to UV spectra and irradiation timing. Kor. J. Hort. Sci. Technol. 27:12–17.
Kim, H.H., G.D. Goins, R.M. Wheeler, and J.C. Sager. 2004a. Green-light supplementation for enhanced lettuce growth under red-and blue-light-emitting diodes. HortScience 39:1617–1622.
Kim, S.J., E.J. Hahn, J.W. Heo, and K.K. Paek. 2004b. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci. Hortic. 101:143–151.
Lee, M.J., J.E. Son, and M.M. Oh. 2013. Growth and phenolic content of sowthistle grown in a closed-type plant production system with a UV-A or UV-B lamp. Hort. Environ. Biotechnol. 54:492–500.
Li, H., C. Tang, and Z. Xu. 2013. The effects of different light qualities on rapeseed (Brassica napus L.) plantlet growth and morphogenesisin vitro. Sci. Hortic. 150:117–124.
Li, Q. and C. Kubota. 2009. Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environ. Expt. Bot. 67:59–64.
Li, S., N.C. Rajapakse, R.E. Young, and R. Oi. 2000. Growth responses of chrysanthemum and bell pepper transplants to photoselective plastic films. Sci. Hortic. 84:215–225.
Lin, K.H., M.Y. Huang, W.D. Huang, M.H. Hsu, Z.W. Yang, and C.M. Yang. 2013. The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Sci. Hortic. 150:86–91.
Maxwell, K. and G.N. Johnson. 2000. Chlorophyll fluorescence-a practical guide. J. Expt. Bot. 51:659–668.
Rockwell, N.C., Y.S. Su, and J.C. Lagarias. 2006. Phytochrome structure and signaling mechanisms. Ann. Rev. Plant Biol. 57:837–858.
Shibuya, T., R. Endo, Y. Kitamura, Y. Kitaya, and N. Hayashi. 2010. Potential photosynthetic advantages of cucumber (Cucumis sativus L.) seedlings grown under fluorescent lamps with high red:far-red light. HortScience 45:553–558.
Shibuya, T., K. Itagaki, M. Tojo, R. Endo, and Y. Kitaya. 2011. Fluorescent illumination with high red-to-far-red ratio improves resistance of cucumber seedlings to powdery mildew. HortScience 46:429–431.
Son, K.H., J.H. Park, D. Kim, and M.M. Oh. 2012. Leaf shape index, growth, and phytochemicals in two leaf lettuce cultivars grown under monochromatic light-emitting diodes. Kor. J. Hort. Sci. Technol. 30:664–672.
Son, K.H. and M.M. Oh. 2013. Leaf shape, growth and antioxidant phenolic compounds of two lettuce cultivars grown under various combinations of blue and red light-emitting diodes. HortScience 48:988–995.
Stutte, G.W. 2009. Light-emitting diodes for manipulating the phytochrome apparatus. HortScience 44:231–234.
Vázquez-Ramos, J.M. and M.P. Sánchez. 2003. The cell cycle and seed germination. Seed Sci. Res. 13:113–130.
Weijschede, J., K. Antonise, H. Caluwe, H. Kroon, and H. Huber. 2008. Effects of cell number and cell size on petiole length variation in a stoloniferous herb. Am. J. Bot. 95:41–49.
Yamada, A., T. Tanigawa, T. Suyama, T. Matsuno, and T. Kunitake. 2009. Red:far-red light ratio and far-red light integral promote or retard growth and flowering in Eustoma grandiflorum (Raf.) Shinn. Sci. Hortic. 120:101–106.
Yang, Z.Q., Y.X. Li, J.B. Zhang, J. Zhang, J. Zhu, L.L. Gu, and B. Zhang. 2013. Effects of the red:far-red light ratio on photosynthetic characteristics of greenhouse cut Chrysanthemum. Hortic. Sci. (Prague) 40:40–43.
Zakir, H.M. 2007. Light emitting diodes increase phenolics of buckwheat (Fagopyrum esculentum) sprouts. J. Plant Interactions 2:71–78.
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Lee, MJ., Park, SY. & Oh, MM. Growth and cell division of lettuce plants under various ratios of red to far-red light-emitting diodes. Hortic. Environ. Biotechnol. 56, 186–194 (2015). https://doi.org/10.1007/s13580-015-0130-1
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DOI: https://doi.org/10.1007/s13580-015-0130-1