Abstract
Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.
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Ko, SM., Sun, HJ., Oh, M.J. et al. Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor . Hortic. Environ. Biotechnol. 52, 511–515 (2011). https://doi.org/10.1007/s13580-011-0007-x
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DOI: https://doi.org/10.1007/s13580-011-0007-x