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Immobilization of a cold-adaptive recombinant Penicillium cyclopium lipase on modified palygorskite for biodiesel preparation

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Abstract

A Pichia pastoris recombinant cold-adaptive lipase (rePcLip) from Penicillium cyclopium was successfully immobilized on modified palygorskite to develop a robust biocatalytic system for biodiesel production from soybean oil. The optimum immobilization conditions were addition amount of 6 g rePcLip to 10 g modified palygorskite, pH 7.0, and binding time 5.0 h. Under these optimal conditions, it reached a transesterification activity of 445.2 U/g (palygorskite with well-immobilized rePcLip, PLG-rePcLip). For biodiesel preparation, the optimized reaction conditions were 20 g soybean oil dissolved in 12 mL t-butanol solvent, 3.7 mL methanol (molar ratio of methanol to oil was 4:1), 0.6 mL water, and 1.6 g PLG-rePcLip at 25 °C and a shaking speed of 200 rpm. The methanol was supplemented at a frequency of 4 times in 48 h (at 0, 7, 16, and 29 h) by adding quarters of methanol that kept 4:1 molar ratio of total methanol to oil. After a 48-h reaction, the transesterification ratio was 93.2%. After reusing immobilized rePcLip for 8 cycles, the residual activity and biodiesel yield were 71% and 65%, respectively. As a non-commercial cold-adaptive biocatalyst, rePcLip immobilized on palygorskite should be a promising alternative for biodiesel production.

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Funding

This work was supported by the Huai’an Science and Technology (Guiding) Project (No. HABZ201706), the Open Project of Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration (No. JPELBCPL2014003), the Natural Science Research Project of Huaiyin Institute of Technology (No. 15HGZ007), and the Key Laboratory of Regional Resource Exploitation and Medicinal Research Project Fund (No. LPRK201905).

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Authors and Affiliations

  1. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, 223003, People’s Republic of China

    Zhongbiao Tan, Jia Zhou, **angqian Li, Shiying Ren & Muhammad Bilal

  2. Key Laboratory of Regional Resource Exploitation and Medicinal Research, School of Chemical Engineering, Huaiyin Institute of Technology, Huai’an, 223003, People’s Republic of China

    Qinghong You

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  1. Zhongbiao Tan
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  2. Jia Zhou
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  3. **angqian Li
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  4. Shiying Ren
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  5. Qinghong You
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Correspondence to Zhongbiao Tan or **angqian Li.

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Tan, Z., Zhou, J., Li, X. et al. Immobilization of a cold-adaptive recombinant Penicillium cyclopium lipase on modified palygorskite for biodiesel preparation. Biomass Conv. Bioref. 12, 5317–5328 (2022). https://doi.org/10.1007/s13399-021-02006-7

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  • DOI: https://doi.org/10.1007/s13399-021-02006-7

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