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The effects of superparamagnetic iron oxide nanoparticles-labeled mesenchymal stem cells in the presence of a magnetic field on attenuation of injury after heart failure

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Abstract

Migration of stem cells after transplantation reduces their therapeutic effects. In this study, we hypothesized that superparamagnetic iron oxide nanoparticles (SPION)-labeled mesenchymal stem cells (MSCs) in the presence of magnetic field may have a capability to increase regenerative ability after heart failure (HF). A rat model of ISO (isoproterenol)-HF was established to investigate the effects of SPION-labeled MSCs on tissue regeneration in the presence and absence of magnetic field. Hydrodynamic size, shape, and formation of chemical bonds between SPION and polyethylene glycol (PEG) were measured using dynamic light scattering (DLS), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). The MRI was used to monitor SPION-labeled MSCs in vivo. Cell and tissue uptake of nanoparticles were determined by Prussian blue staining, atomic absorption spectroscopy (AAS), and inductively coupled plasma spectroscopy (ICP). Purity of the MSCs, heart function, myocardial fibrosis, and histologic damage were evaluated using flow-cytometry, echocardiography, Masson’s trichrome, and H&E staining respectively. Various spectroscopic and microscopic analyses revealed that hydrodynamic size of SPION was 40 ± 2 and their shape was spherical. FTIR confirmed the presence of PEG on the surface of nanoparticles. The presence of magnetic field significantly increased cell homing. Highly purified MSCs population was detected by flow-cytometry. Using SPION-labeled MSCs in the presence of magnetic field markedly improved heart function and myocardial hypertrophy and reduced fibrosis (p < 0.05). Collectively, our results demonstrated that SPION-labeled MSCs in the presence of magnetic field might contribute to regeneration after HF.

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Abbreviations

SPION:

Superparamagnetic iron oxide nanoparticles

MSCs:

Mesenchymal stem cells

hAMSCs:

Human amniotic mesenchymal stromal cells

DLS:

Dynamic light scattering

HF:

Heart failure

ISO:

Isoproterenol

ISO-HF:

ISO-induced heart failure

TEM:

Transmission electron microscopy

FTIR:

Fourier-transform infrared spectroscopy

2D:

Two dimensional

PEG:

Polyethylene glycol

ICP:

Inductively coupled plasma spectroscopy

MRI:

Magnetic resonance imaging

AAS:

Atomic absorption spectroscopy

VSM:

Vibrating sample magnetometer

EF:

Ejection fraction

FS:

Fractional shortening

LVDd:

Left ventricular diameter in diastole

LVDs:

Left ventricular diameter in systole

H&E:

Hematoxylin and eosin

FACS:

Fluorescence-activated cell sorting

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Fetal bovine serum

MDT:

Magnetic drug targeting

MTE:

Magnetic tissue engineering

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Funding

This study was supported financially by Physiology Research Center of Iran, University of Medical Sciences.

Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Islamic Azad University Tehran Medical Sciences, Tehran, Iran

    Maryam Naseroleslami

  2. Physiology Research Center, Iran university of Medical Sciences, Tehran, Iran

    Nahid Aboutaleb

  3. Department of Physiology, Iran university of Medical Sciences, Tehran, Iran

    Nahid Aboutaleb

  4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Kazem Parivar

Authors
  1. Maryam Naseroleslami
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  2. Nahid Aboutaleb
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Corresponding author

Correspondence to Nahid Aboutaleb.

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Ethical approval

Animal Ethical Committee of Iran University of Medical Sciences approved all protocols and surgical procedures. The animals were housed five per cage in a room with controlled temperature, on 12-h:12-h light:dark schedule, moisture (40–60%) with free access to standard food and water.

Informed consent

The amniotic membranes were provided by Shahid Akbar Abadi Hospital upon informed consent of the participants in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Naseroleslami, M., Aboutaleb, N. & Parivar, K. The effects of superparamagnetic iron oxide nanoparticles-labeled mesenchymal stem cells in the presence of a magnetic field on attenuation of injury after heart failure. Drug Deliv. and Transl. Res. 8, 1214–1225 (2018). https://doi.org/10.1007/s13346-018-0567-8

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  • DOI: https://doi.org/10.1007/s13346-018-0567-8

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