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miR-133b acts as a tumor suppressor and negatively regulates FGFR1 in gastric cancer

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Tumor Biology

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that negatively regulate protein expression by binding protein-coding mRNAs and repressing translation. Accumulating evidence suggests that miRNAs are involved in cancer development and progression, acting as either tumor suppressors or oncogenes. Intriguingly, it has been shown that miR-133b was significantly downregulated in several types of cancers. However, its role and relevance in gastric cancer are still largely unknown. We showed that miR-133b was downregulated in human gastric cancer tissues and cell lines compared with nontumor counterparts by quantitative RT-PCR analysis. Overexpression of miR-133b could inhibit cell proliferation and colony formation of the gastric cancer cell lines MKN-45 and SGC-7901. Bioinformatics analysis indicated two putative miR-133b binding sites in the 3′-untranslated region of fibroblast growth factor receptor 1 (FGFR1) mRNA. In dual-luciferase reporter assay, miR-133b reduced the luciferase activity of Luc-FGFR1-wt, and mutation of miR-133b binding sites abolished the inhibitory effect of miR-133b. In this study, we found that miR-133b reduced the protein but not the mRNA levels of endogenous FGFR1. Furthermore, FGFR1 expression was upregulated in gastric cancer tissues and inversely correlated with miR-133b expression. Finally, knockdown of FGFR1 inhibited the growth of MKN-45 cells in a dose-dependent manner and overexpression of FGFR1 promoted the growth of GES-1 cells. These results indicate that miR-133b targets FGFR1 and inhibits gastric cancer cell growth, suggesting that it may serve as a tumor suppressive target in gastric cancer therapy.

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Funding

This work was supported by the Natural Science Foundation of Jilin Province (201215083) and the Special Foundation for Industrial Technology Research and Development of Jilin Province (2010018-5).

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Authors and Affiliations

  1. Department of General Surgery, The Second Hospital of Jilin University, No.218, Zi Qiang Str, Nan Guan District, Changchun, 130041, Jilin Province, China

    Dacheng Wen, Fujian Ji, Hong Cao, Weidong Jiang, Jiaming Zhu & Xuedong Fang

  2. Department of Ophthalmology, The First Hospital of Jilin University, No. 71, **nmin Avenue, Chaoyang District, Changchun, 130021, Jilin Province, China

    Songhe Li

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  1. Dacheng Wen
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Correspondence to Jiaming Zhu or Xuedong Fang.

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Wen, D., Li, S., Ji, F. et al. miR-133b acts as a tumor suppressor and negatively regulates FGFR1 in gastric cancer. Tumor Biol. 34, 793–803 (2013). https://doi.org/10.1007/s13277-012-0609-7

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