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Coating of Laponite on PLA Nanofibrous for Bone Tissue Engineering Application

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Abstract

In this study, electrospun polylactic acid (PLA) nanofibers were coated with osteoinductive Laponite (LAP) nanoplatelets and used as scaffolds for bone tissue engineering. The LAP nanoplatelets were physically crosslinked with high molecular weight polyethylene oxide (PEO) to make a layer of LAP/PEO network around PLA nanofibers with a thickness of 45–175 nm that was controllable through adjusting the concentration of the LAP/PEO solution (0.4, 0.8, and 1.2 wt%). The LAP/PEO layer improved the surface hydrophilicity (water contact angle from 111° to 26°) and thus the cell attachment and proliferation (p ≤ 0.05) by the scaffolds. The LAP nanoplatelets coated on the surface of PLA nanofibers could induce osteodifferentiation on human mesenchymal stem cells (hMSCs) by increasing the ALKALINE PHOSPHATASE (ALP) activity. The expression of specific osteogenic genes (ALP and OSTEONECTIN) at the transcription level (p ≤ 0.001) for the seeded hMSCs proved the osteoinductive effect of coated LAP nanoplatelets for the differentiation of stem cells to osteoblasts without using any external osteogenic inducers. The LAP-coated nanofibers can be used as an excellent scaffold for bone tissue engineering to provide an appropriate environment for direct bone tissue engineering.

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Authors and Affiliations

  1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Zahra Orafa & Shiva Irani

  2. Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Tehran, Iran

    Ali Zamanian

  3. Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstraße 68, 14476, Potsdam, Germany

    Hadi Bakhshi

  4. Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Habib Nikukar

  5. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Habib Nikukar

  6. Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Behafarid Ghalandari

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  1. Zahra Orafa
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  2. Shiva Irani
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  3. Ali Zamanian
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Correspondence to Shiva Irani.

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Orafa, Z., Irani, S., Zamanian, A. et al. Coating of Laponite on PLA Nanofibrous for Bone Tissue Engineering Application. Macromol. Res. 29, 191–198 (2021). https://doi.org/10.1007/s13233-021-9028-1

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  • DOI: https://doi.org/10.1007/s13233-021-9028-1

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