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SPION loaded poly(L-lysine)/hyaluronic acid micelles as MR contrast agent and gene delivery vehicle for cancer theranostics

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Abstract

In this work we evaluated the ability of a SPION (Superparamagnetic iron oxide nanoparticle) loaded micelle system to safely and efficiently transfer foreign DNA into cells with the help of poly(L-lysine) (PLL) as DNA-carrier. Average particle size of PLL coated hyaluronic acid (HA) loaded SPION (PLL-HA-SPION) micelle was approximately 200 nm in size with spherical morphology. PLL-HA-SPION micelle was found to be nontoxic to the NIH3T3 (murine fibroblast cells) and the in vitro uptake was also tested in CT-26 (murine colon cancer cells) by prussian blue staining. Agarose gel retardation study showed the efficient binding of PLL-HA-SPION micelle with plasmid DNA (pLuc). The formed PLL-HA-SPION/pLuc complex could be efficiently transfected into CT-26 cell line. Finally, MR imaging was also used to check uptake property of SPION loaded in PLL-HA-SPION/DNA complex in vitro. The result showed that the PLL-HA-SPION micelle can be applied to MR imagingguided gene therapy in vivo and it is also advantageous for using this system for the magnetic hyperthermia based cancer therapy.

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Authors and Affiliations

  1. Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 61469, Korea

    Reju George Thomas & Yong Yeon Jeong

  2. Department of Biomedical Science, Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, 61469, Korea

    Muthunarayanan Muthiah & In-Kyu Park

  3. DKC corporation (BioActs), Incheon, South Korea

    MyeongJu Moon

  4. Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA

    Muthunarayanan Muthiah

Authors
  1. Reju George Thomas
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  2. Muthunarayanan Muthiah
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  3. MyeongJu Moon
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  4. In-Kyu Park
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  5. Yong Yeon Jeong
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Correspondence to Yong Yeon Jeong.

Additional information

Acknowledgments: We would like to thank Basic Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Future Planning (2015R1A2A2A01007798) for providing financial support.

These authors equally contributed to this work.

The image from this article is used as the cover image of the Volume 25, Issue 5.

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Thomas, R.G., Muthiah, M., Moon, M. et al. SPION loaded poly(L-lysine)/hyaluronic acid micelles as MR contrast agent and gene delivery vehicle for cancer theranostics. Macromol. Res. 25, 446–451 (2017). https://doi.org/10.1007/s13233-017-5053-5

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  • DOI: https://doi.org/10.1007/s13233-017-5053-5

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