Abstract
The lateral flow immunoassay called “rapid test” has been widely used for medical diagnosis, environmental monitoring, forensic tests, and so on. This method carries out whole immunoassay process including washing and signal reporting simply by drop** a sample to a test strip. During the capillary flow of a sample on the test strip, colored signal bands are produced by the targeted antigen-antibody interactions. In this study, a highly sensitive rapid test was developed by applying a chemiluminescence enzyme reaction. In this method, a chemiluminescent signal band was produced by using horseradish peroxidase (HRP) labeled antibodies and luminol as a chemiluminescence substrate. The intensity of the chemiluminescent signal band was evaluated by using a charge coupled device (CCD) with an image analysis program. The feasibility of the new rapid test with a chemiluminescent signal band was presented by the detection of horseradish peroxidase (HRP) as a model analyte. The applicability of this new rapid test to sandwich-type immunoassays was demonstrated by the detection of myoglobin, and the sensitivity was determined to be improved as much as 100-fold in compared to the conventional rapid test based on colored gold-colloids.
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Yousif, A., Anderson, Y.J., Chard-Bergstrom, C. & Kapil, S. Development, Evaluation, and Application of lateral-flow immunoassay (Immunochromatography) for detection of rotavirus in bovine fecal samples. Clin. Diagn. Lab. Immunol. 9, 723–724 (2002).
Ferris, N.P. et al. Development and laboratory validation of a lateral flow device for the detection of foot- and-mouth disease virus in clinical samples. J. Virol. Methods 155, 10–17 (2009).
Nadanaciva, S. et al. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis. J. Immunol. Methods 343, 1–12 (2009).
Wang, S., Zhang, C., Wang, J. & Zhang, Y. Development of colloidal gold-based flow-through and lateral-flow immunoassays for the rapid detection of the insecticide carbaryl. Anal. Chim. Acta 546, 161–166 (2005).
Harvey, M.A., Audette, C.A. & McDonogh, R. The use of microporous polymer membranes in immunoassays. IVD Technol. 2, 34–40 (1996).
Oku, Y. et al. Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection. J. Immunol. Methods 258, 73–84 (2001).
Qian, S. & Haim, H.H. Analysis of lateral flow biodetectors: competitive format. Anal. Biochem 326, 211–224 (2004).
Snowden, K. & Hommel, M. Antigen detection immunoassay using dipsticks and colloidal dyes. J. Immunol. Methods 140, 57–65 (1991).
Dyke, K.V., Dyke, C.V. & Woodfork, K. Luminescence Biotechnology. CRC Press, NY, USA (2002).
Lonnberg, M. & Carlsson, J. Membrane assisted isoform immunoassay: A rapid method for the separation and determination of protein isoforms in an integrated immunoassay. J. Immunol. Methods 246, 25–36 (2000).
Choi, S. et al. A rapid, simple measurement of human albumin in whole blood using a fluorescence immunoassay (I). Clin. Chim. Acta 339, 147–156 (2004).
Sippy, N., Luxton, R., Lewis, R.J. & Cowell, D.C. Rapid electrochemical detection and identification of catalase positive micro-organisms. Biosens. Bioelectron. 18, 741–749 (2003).
Fernandez-Sanchez, C., Gallardo-Soto, A.M., Rawson, K., Nilsson, O. & McNeil, C.J. Quantitative impedimetric immunosensor for free and total prostate specific antigen based on a lateral flow assay format. Electrochem. Commun. 6, 138–143 (2004).
Chan, C.P.Y. et al. Development of a quantitative lateral-flow assay for rapid detection of fatty acidbinding protein. J. Immunol. Methods. 279, 91–100 (2003).
Xu, Z., Guo, Z. & Dong, S. Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris (2,2′-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material. Biosens. Bioelectron. 21, 455–461 (2005).
Zheng, X. & Zhang, Z. Flow-injection chemiluminesence detecting sulfite with in situ eletrogenerated Mn3+ as the oxidant. Sensor. Actuat. B-Chem. 84, 142–147 (2002).
Penttila, K. et al. Myoglobin, creatine kinase MB isoforms and creatine kinase MB mass in early diagnosis of myocardial infarction in patients with acute chest pain. Clin. Biochem. 35, 647–653 (2002).
Plebani, M. & Zaninotto, M. Diagnostic strategies using myoglobin measurement in myocardial infarction. Clin. Chim. Acta 272, 69–77 (1998).
Jurlander, B., Clemmensen, P., Wagner, G.S. & Grande, P. Very early diagnosis and risk stratification of patients admitted with suspected acute myocardial infarction by the combined evaluation of a single serum value of cardiac troponin-T, myoglobin, and creatine kinase MBmass. Eur. Heart. J. 21, 382–389 (2000).
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Kim, HS., Ko, H., Kang, MJ. et al. Highly sensitive rapid test with chemiluminescent signal bands. BioChip J 4, 155–160 (2010). https://doi.org/10.1007/s13206-010-4211-z
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DOI: https://doi.org/10.1007/s13206-010-4211-z