Abstract
The newly isolated Trametes polyzona PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by Trametes polyzona PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h. The degradation metabolites showed the absence of toxic compounds. Microbial viability tests using E. coli and B. subtilis revealed that the treated phenanthrene was less toxic than untreated phenanthrene. Phytotoxicity and genotoxicity tests, using Vigna radiata and Allium cepa, indicated that the treated phenanthrene was less toxic to the plants. No mutagenic activity was found in the Ames test. The crude laccase from Trametes polyzona PBURU 12 was demonstrated as a potential tool for the biodegradation of PAHs (phenanthrene), with low toxic effects after the degradation.
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Acknowledgements
The authors thank the East Kalimantan Provincial Government, Indonesia for supporting a grant under the program East Kalimantan Excellent Scholarship for the Ph.D. and the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University for Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University for financial support.
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Wulandari, R., Lotrakul, P., Punnapayak, H. et al. Toxicity evaluation and biodegradation of phenanthrene by laccase from Trametes polyzona PBURU 12. 3 Biotech 11, 32 (2021). https://doi.org/10.1007/s13205-020-02556-z
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DOI: https://doi.org/10.1007/s13205-020-02556-z