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Separation/preconcentration and determination of quercetin in food samples by dispersive liquid–liquid microextraction based on solidification of floating organic drop -flow injection spectrophotometry

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Abstract

A new dispersive liquid–liquid microextraction based on solidification of floating organic drop (DLLME-SFOD)-flow injection spectrophotometry (FI) method for the separation and preconcentration of trace amounts of quercetin was developed. 1-Undecanol and methanol was used as the extraction and disperser solvent, respectively. The factors influencing the extraction by DLLME-SFOD such as the volume of the extraction and disperser solvents, pH and concentration of salt were optimized. The optimal conditions were found to be; volume of the extraction solvent, 80 μL; the volume of the disperser solvent, 100 μL; and the pH of the sample, 3. The linear dynamic range and detection limit were 5.0 × 10−8–5.0 × 10−7 mol L−1 and 1 × 10−8 mol L−1, respectively. The relative standard deviation (R.S.D.) at 6.1 × 10−8 mol L−1 level of quercetin (n = 10) was found to be 2.8 %. The method was successfully applied to the determination of quercetin in the apple, grape, onion and tomato samples.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Yazd University, Yazd, 89195-741, Iran

    Tahereh Asadollahi, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani & Mooud Amirkavei

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  1. Tahereh Asadollahi
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  2. Shayessteh Dadfarnia
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  3. Ali Mohammad Haji Shabani
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  4. Mooud Amirkavei
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Correspondence to Shayessteh Dadfarnia.

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Asadollahi, T., Dadfarnia, S., Haji Shabani, A.M. et al. Separation/preconcentration and determination of quercetin in food samples by dispersive liquid–liquid microextraction based on solidification of floating organic drop -flow injection spectrophotometry. J Food Sci Technol 52, 1103–1109 (2015). https://doi.org/10.1007/s13197-013-1077-9

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  • DOI: https://doi.org/10.1007/s13197-013-1077-9

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