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In-vitro evaluation of bioactive compounds, anti-oxidant, lipid peroxidation and lipoxygenase inhibitory potential of Citrus karna L. peel extract

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Abstract

Many medicinal plants have been studied for their antioxidant and their pharmacological activity. Citrus species were well documented as potential antioxidant based therapy for cancer, inflammation, heart disease. Citrus seeds and peels have been shown to possess high antioxidant activity. Therefore, the present study to explore the antioxidant and lipid peroxidation & lipoxygenase inhibitory action of Citrus karna peel extracts were undertaken. Extraction was performed with different solvents of increasing polarity and yield was calculated. Peel extracts were also analyzed for the presence of phenols, flavonoids, vitamin C, and carotenoids. Then the Citrus karna peel extracts were evaluated for the antioxidant and lipid peroxidation & lipoxygenase inhibitory action In-Vitro. In further, the quantification of hesperidin and naringin was carried out by HPLC-DAD method. The results indicated the presence of phenols, flavonoids, vitamin C, carotenoids, hesperidin and naringin in Citrus karna peel extracts with maximum yield of (3.91% w/w). Citrus karna peel extracts were also found to have potential antioxidant and lipid peroxidation & lipoxygenase inhibitory action. Therefore, Citrus karna peel extracts could be used for the future therapeutic medicine due to presence of potential bioactive compounds.

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References

  • Ajila CM, Naidu KA, Bhat SG, Prasada Rao UJS (2007) Bioactive compounds and antioxidant potential of mango peel extract. Food Chem 105:982–988

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  • Cano A, Medina A, Bermejo A (2008) Bioactive compounds in different citrus varieties. Discrimination among cultivars. J Food Compos Anal 21:377–381

    Article  CAS  Google Scholar 

  • Chen Y, Wang J, Wan D (2010) Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry. Phcog Mag 6:259–263

    Article  CAS  Google Scholar 

  • Das K, Samanta TT, Samanta P, Nandi DK (2010) Effect of extract of Withania Somnifera on dehydration-induced oxidative stress-related uremia in male rats. Saudi J Kidney Dis Transpl 21:75–80

    Google Scholar 

  • Dhuique-Mayer C, Caris-Veyrat C, Ollitrault P, Curk F, Amiot MJ (2005) Varietal and interspecific influence on micronutrient contents in Citrus from the Mediterranean area. J Agric Food Chem 53:2140–2145

    Article  CAS  Google Scholar 

  • Eleni B, Dailianis S (2010) Involvement of Na(+)/H(+) exchanger and respiratory burst enzymes NADPH oxidase and NO synthase, in Cd-induced lipid peroxidation and DNA damage in haemocytes of mussels. Comp Biochem Physiol C Toxicol Pharmacol 152:346–352

    Article  Google Scholar 

  • Hamdan D, El-Readi MZ, Nibret E, Sporer F, Farrag N, El-Shazly A, Wink M (2010) Chemical composition of the essential oils of two Citrus species and their biological activities. Pharmazie 65:141–147

    CAS  Google Scholar 

  • Harborne JB (1973) Phytochemical methods. Chapman and Hall Ltd., London, pp 49–188

    Google Scholar 

  • Joshipura KJ, Hu FB, Manson JE, Stampfer MJ, Rimm EB, Speizer FE, Colditz G, Ascherio A, Rosner B, Spiegelman D, Willett WC (2001) The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med 34:1106–1114

    Article  Google Scholar 

  • Kanaze FI, Kokkalou E, Georgarakis M, Niopas I (2004) A validated solid-phase extraction HPLC method for the simultaneous determination of the Citrus flavanone aglycones hesperetin and naringenin in urine. J Pharmaceut Biomed Anal 36:175–181

    Article  CAS  Google Scholar 

  • Keereetaweep J, Kilaru A, Feussner I, Venables BJ, Chapman KD (2010) Lauroylethanolamide is a potent competitive inhibitor of lipoxygenase activity. FEBS Lett 584:3215–3222

    Article  CAS  Google Scholar 

  • Lee MY, Lee NH, Seo CS, Lee JA, Jung D, Kim JH, Shin HK (2010) Alpinia katsumadai seed extract attenuate oxidative stress and asthmatic activity in a mouse model of allergic asthma. Food Chem Toxicol 48:1746–1752

    Article  CAS  Google Scholar 

  • Luzia DMM, Jorge N (2011) Fatty acids profile and alteration of lemon seeds extract (Citrus limon) added to soybean oil under thermoxidation. J Food Sci Tech. doi:10.1007/s13197-011-0411-3

  • Miller DM, Aust SD (1989) Studies of ascorbate–dependent, ironcatalyzed lipid peroxidation. Arch Biochem Biophys 271:113–119

    Article  CAS  Google Scholar 

  • Muthuraman A, Sood S (2010a) Pharmacological evaluation of tacrolimus (FK-506) on ischemia reperfusion induced vasculatic neuropathic pain in rats. J Brachial Plex Peripher Nerve Inj 5:13–23

    Article  Google Scholar 

  • Muthuraman A, Sood S (2010b) Antisecretory, antioxidative and antiapoptotic effects of montelukast on pyloric ligation and water immersion stress induced peptic ulcer in rat. Prostaglandins Leukot Essent Fatty Acids 83:55–60. doi:10.1016/j.plefa.2010.01.003

    Article  CAS  Google Scholar 

  • Muthuraman A, Diwan V, Jaggi AS, Singh N, Singh D (2008) Ameliorative effects of Ocimum sanctum in sciatic nerve transection-induced neuropathy in rats. J Ethnopharmacol 120:56–62

    Article  CAS  Google Scholar 

  • Oboh G, Ademosun AO (2011) Characterization of the antioxidant properties of phenolic extracts from some citrus peels. J Food Sci Tech. doi:10.1007/s13197-010-0222-y

  • Pereira DM, Valentao P, Pereira JA, Andrade PB (2009) Phenolics: from chemistry to biology. Molecules 14:2202–2211

    Article  CAS  Google Scholar 

  • Rey A, Bahamonde A, Casas JA, Rodríguez JJ (2010) Selectivity of hydrogen peroxide decomposition towards hydroxyl radicals in catalytic wet peroxide oxidation (CWPO) over Fe/AC catalysts. Water Sci Tech 61:2769–2778

    Article  CAS  Google Scholar 

  • Ruch RJ, Cheng SJ, Klaunig JE (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10:1003–1008

    Article  CAS  Google Scholar 

  • Shobana S, Naidu KA (2000) Antioxidant activity of selected Indian spices. Prostaglandins Leukot Essent Fatty Acids 62:107–110

    Article  CAS  Google Scholar 

  • Smith SS, Reyes JR, Arbon KS, Harvey WA, Hunt LM, Heggland SJ (2009) Cadmium-induced decrease in RUNX2 mRNA expression and recovery by the antioxidant N-acetylcysteine (NAC) in the human osteoblast-like cell line, Saos-2. Toxicol Vitr 23:60–66

    Article  CAS  Google Scholar 

  • Sood S, Arora B, Bansal S, Muthuraman A, Gill NS, Arora R, Bali M, Sharma PD (2009) Antioxidant, anti-inflammatory and analgesic potential of the Citrus decumana L. peel extract. Inflammopharmacol 17:267–274

    Article  Google Scholar 

  • Sood S, Muthuraman A, Arora B, Bansal S, Bali M, Sharma PD (2010) Potential effect of Citrus decumana extract on stress induced peptic ulcer in rat. Lat Am J Pharm 29:52–56

    Google Scholar 

  • Steel RGD, Torrie JH (1980) Principles and procedures of statistics. McGraw, New York

    Google Scholar 

  • Watcho P, Stavniichuk R, Ribnicky DM, Raskin I, Obrosova IG (2010) High-fat diet-induced neuropathy of prediabetes and obesity: effect of PMI-5011, an ethanolic extract of Artemisia dracunculus L. Mediat Inflamm 2010:1–10

    Article  Google Scholar 

  • Yen GC, Chen HY (1995) Antioxidant activity of various tea extracts in relation to their anti-mutagenicity. J Agric Food Chem 43:27–32

    Article  CAS  Google Scholar 

  • Yoshida H, Takamura N, Shuto T, Ogata K, Tokunaga J, Kawai K, Kai H (2010) The Citrus flavonoids hesperetin and naringenin block the lipolytic actions of TNF-alpha in mouse adipocytes. Biochem Biophys Res Commun 394:728–732

    Article  CAS  Google Scholar 

Download references

Acknowledgement

Thanks to professor all faculty members of Rayat Institute of Pharmacy for their encouragement and support. We are also grateful to Rayat & Bahra Educational and Research Trust for their unconditional help to carry out this project.

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Authors and Affiliations

  1. Rayat Institute of Pharmacy, Nawanshahr District Near Ropar, Distt Shaheed Bhagat Singh Nagar, Railmajra, Punjab, 144533, India

    Jagdeep Singh, Shailja Sood & Arunachalam Muthuraman

  2. Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, 147002, Punjab, India

    Arunachalam Muthuraman

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  1. Jagdeep Singh
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  2. Shailja Sood
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  3. Arunachalam Muthuraman
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Correspondence to Arunachalam Muthuraman.

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Singh, J., Sood, S. & Muthuraman, A. In-vitro evaluation of bioactive compounds, anti-oxidant, lipid peroxidation and lipoxygenase inhibitory potential of Citrus karna L. peel extract. J Food Sci Technol 51, 67–74 (2014). https://doi.org/10.1007/s13197-011-0479-9

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  • DOI: https://doi.org/10.1007/s13197-011-0479-9

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