Abstract
In this study, we developed 27 polymorphic microsatellite loci in Hucho bleekeri, a glacial relict and freshwater resident salmonid fish in China. The number of alleles varied from 2 to 8 for each primer set. The observed and expected heterozygosity ranged from 0.250 to 0.906 and 0.508 to 0.845, respectively. The polymorphic information content ranged from 0.371 to 0.808. These microsatellite loci should be useful to study population genetics, paternity identification, speciation and adaptive evolution of this lineage.
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Acknowledgments
This work was supported by Special Fund for Agro-Scientific Research in the Public Interest (201203086) and open projects for Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China (No. LFBC0802). The fundamental Research Funds for the Central Public Research Institutes (2013JBFM19 and 2014A07XK05). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
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Ke Wang and Shuhuan Zhang have contributed equally to this work.
Appendix: Sequences of H. bleekeri
Appendix: Sequences of H. bleekeri
The orange parts are primers
The highlighted parts are repetitive sequences
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Wang, K., Zhang, S., Wang, D. et al. Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using next-generation sequencing technology. Conservation Genet Resour 7, 263–267 (2015). https://doi.org/10.1007/s12686-014-0353-y
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DOI: https://doi.org/10.1007/s12686-014-0353-y