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Optimization for Production of a Plant Growth Promoting Agent from the Degradation of Chicken Feather Using Keratinase Producing Novel Isolate Bacillus pumilus JYL

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Abstract

Feather is a good source of nitrogen but the highly rigid structure limits its utilization and leads to environmental pollution and resource wastage. Traditional feather degradation processes such as alkali hydrolysis and steam pressure cooking not only destroy the amino acids but also consume large amounts of energy. Therefore, searching for feather degrading bacteria to transform feather waste to nitrogen fertilizer to improve soil and plant growth is needed. And this also will solve the soil nitrogen deficiency problem in China. In this study, a bacteria that could degrade feather and promote plant growth was isolated from soil sample using dilution plate method. And the isolated bacteria was identified as Bacillus pumilus JYL according to its morphology, biochemical characteristics and 16S rRNA sequence. And the maximum keratinase activity (57.14 U/mL) was obtained under optimal conditions of 0.036% FeSO4, 0.025% MgSO4, 0.073% K2HPO4, 0.5% feather with 2% inoculant at pH 6.5 fermented for 36 h at 35 ℃ after optimization using (response surface methodology) RSM. The effect of hydrolyzate on wheat seed germination and growth was studied. The results showed that germination engergy and germination index with 3% feather hydrolyzate addition group was 94.22% and 87.52%, significantly higher than that of the control group (p < 0.05), respectively. The plant height, root length, fresh weight and dry weight in the T2 treatment (added 5 mL feather hydrolysate in 100 g soil) at 60th day were 1.10, 1.16, 1.77 and 1.73 times than that in the CK treatment (without addition of hydrolysate), respectively. And the soil enzyme activities in all treatment groups (T1, T2, T3) significantly increased compared with control group (CK) (p < 0.05). This result will provide a potential utilization method for feather valoration.

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References

  1. **, E., Reddy, N., Zhu, Z.Y.: Graft polymerization of native chicken feathers for thermoplastic applications. J. Agric. Food Chem. 59, 1729–1738 (2011)

    Google Scholar 

  2. Dou, Y., Xue, H., Buning, Z., Ming, H., Guoqiang, Y., Yingde, C.: Preparation and characterization of dialdehyde starch crosslinked feather keratin film for food packaging application. Rsc Adv. 5, 27168–27174 (2015)

    Google Scholar 

  3. Gousterova, A., Braikova, D., Goshev, I., Christov, P., Tishinov, K., Vasileva-Tonkova, E., Haertlé, T., Nedkov, P.: Degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis. Lett. Appl. Microbiol. 40, 335–340 (2005)

    Google Scholar 

  4. Onifade, A.A., Al-Sane, N.A., Al-Musallam, A.A., Al-Zarban, S.: A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol. 66, 1–11 (1998)

    Google Scholar 

  5. Pintubala, K., Subhra, S.R., Kumar, S.S., Thangjam, S.S., Meraj, A.A., Narendra, P., Ngachan, S.V.: Transforming chicken feather waste into feather protein hydrolysate using a newly Iisolated multifaceted keratinolytic bacterium Chryseobacterium sediminis RCM-SSR-7. Waste Biomass Valor. 10, 1–11 (2019)

    Google Scholar 

  6. Williams, C.M., Lee, C.G., Garlich, J.D., Shih, J.C.H.: Evaluation of a bacterial feather fermentation product, feather-lysate, as a feed protein. Poultry Sci. 70, 85–94 (1991)

    Google Scholar 

  7. Yamamura, S., Morita, Y., Hasan, Q., Yokoyama, K., Tamiya, E.: Keratin degradation: a cooperative action of two enzymes from Stenotrophomonas sp. Biochem. Biophys. Res. Commun. 294, 1138–1143 (2002)

    Google Scholar 

  8. Jain, R., Jain, A., Rawat, N., Nair, M., Gumashta, R.: Feather hydrolysate from Streptomyces sampsonii GS 1322: a potential low cost soil amendment. J. Biosci. Bioeng. 121, 672–677 (2016)

    Google Scholar 

  9. **ha, J., Omi, L., Youngdong, J., Jeongdo, K., Nari, L., Chungyeol, L., Hongjoo, S.: Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity. Process Biochem. 45, 1738–1745 (2010)

    Google Scholar 

  10. Kumar, A.G., Swarnalatha, S., Gayathri, S., Nagesh, N., Sekaran, G.: Characterization of an alkaline active-thiol forming extracellular serine keratinase by the newly isolated Bacillus pumilus. J. Appl. Microbiol. 104, 411–419 (2008)

    Google Scholar 

  11. Okoroma, E.A., Hemda, G., Abiola, O.O., Diane, P.: Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather. Int. Biodeter. Biodegr. 74, 54–60 (2012)

    Google Scholar 

  12. Pillai, P., Mandge, S., Archana, G.: Statistical optimization of production and tannery applications of a keratinolytic serine protease from Bacillus subtilis P13. Process Biochem. 46, 1110–1117 (2011)

    Google Scholar 

  13. Brandelli, A., Daroit, D.J., Riffel, A.: Biochemical features of microbial keratinases and their production and applications. Appl. Microbiol. Biotechnol. 85, 1735–1750 (2010)

    Google Scholar 

  14. Choi, J.M., Nelson, P.V.: Develo** a slow-release nitrogen fertilizer from organic sources: using poultry feathers. J. Am. Soc. Hortic. Sci. 121, 634–638 (1996)

    Google Scholar 

  15. Vesela, M., Friedrich, J.: Amino acid and soluble protein cocktail from waste keratin hydrolysed by a fungal keratinase of Paecilomyces marquandii. Biotechnol. Bioprocess. Eng. 14, 84–90 (2009)

    Google Scholar 

  16. Paul, T., Halder, S.K., Das, A., Bera, S., Maity, C.: Exploitation of chicken feather waste as a plant growth promoting agent using keratinase producing novel isolate Paenibacillus woosongensis. Biocatal. Agric. Biotechnol. 2, 50–57 (2013)

    Google Scholar 

  17. Jeong, J.H., Lee, O.M., Jeon, Y.D., Kim, J.D., Lee, N.R.: Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth promoting activity. Process Biochem. 45, 1738–1745 (2010)

    Google Scholar 

  18. Lateef, A., Oloke, J.K., Gueguim Kana, E.B., Sobowale, B.O., Ajao, S.O., Bello, B.Y.: Keratinolytic activities of a new feather-degrading isolate of Bacillus cereus LAU 08 isolated from Nigerian soil. Int. Biodeter. Biodegr. 64, 162–165 (2010)

    Google Scholar 

  19. Glickman, E., Dessaux, Y.: A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl. Environ. Microbiol. 61, 793–796 (1995)

    Google Scholar 

  20. Schwyn, B., Neilands, J.B.: Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47–52 (1987)

    Google Scholar 

  21. Penrose, D.M., Glick, B.R.: Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol. Plant. 118, 10–15 (2003)

    Google Scholar 

  22. Kumar, V., Narula, N.: Solubilization of inorganic phosphates and growth emergence of wheat as affected by Azotobacter chroococcum mutants. Biol. Fertil. Soils. 28, 301–305 (1999)

    Google Scholar 

  23. Singh, A.V., Agarwal, A., Goel, R.: Comparative phosphates solubilization efficiency of two bacterial isolates and their effect on Cicer arietinum seeds in indigenous and alternative soil system. Environ. Ecol. 28, 1979–1983 (2010)

    Google Scholar 

  24. Cappuccino, J.G., Sherman, N.: Microbiology a Laboratory Manual. Benjamin Cummings Science Publishing, California (1996)

    Google Scholar 

  25. Madigan, M.T., Imhoff, J.F.: Phylum BXIII. Firmicutes. In: Boone, D.R., Castenholz, R.W., Garrity, G.M. (eds.) Bergey’s Manual® of Systematic Bacteriology. Springer, New York (2001)

    Google Scholar 

  26. Hosek, J., Svastova, P., Moravkova, M., Pavlik, I., Bartos, M.: Methods of mycobacterial DNA isolation from different biological material: a review. Vet Med. 51, 180–192 (2006)

    Google Scholar 

  27. Rahman, R.N., Mahamad, S., Salleh, A.B., Basri, M.: A new organic solvent tolerant protease from Bacillus pumilus 115b. J. Ind. Microbiol. Biot. 34, 509–517 (2007)

    Google Scholar 

  28. Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S.: MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729 (2013)

    Google Scholar 

  29. Gradisar, H., Kern, S., Friedrich, J.: Keratinase of Doratomyces microsporus. Appl. Microbiol. Biotechnol. 53, 196–200 (2000)

    Google Scholar 

  30. Plackett, R.L., Burman, J.P.: The design of optimum multifactorial experiments. Biometrika 33, 305–325 (1946)

    MathSciNet  MATH  Google Scholar 

  31. Nustorova, M., Braikova, D., Gousterova, A., Vasileva-Tonkova, E., Nedkov, P.: Chemical, microbiological and plant analysis of soil fertilized with alkaline hydrolysate of sheep’s wool waste. World J. Microb. Biot. 22, 383–390 (2006)

    Google Scholar 

  32. Wang, Y.F., Ma, Z.T., Wang, X.W., Sun, Q.R., Dong, H.Q., Wang, G.S., Chen, X.S., Yin, C.M., Han, Z.H., Mao, Z.Q.: Effects of biochar on the growth of apple seedlings, soil enzyme activities and fungal communities in replant disease soil. Sci. Hortic-Amsterdam. (2019). https://doi.org/10.1016/j.scienta.2019.108641

    Article  Google Scholar 

  33. Zhang, Z., Chen, Q., Yin, C., Shen, X., Chen, X., Sun, H., Gao, A.N., Mao, Z.: The effects of organic matter on the physiological features of Malus hupehensis seedlings and soil properties under replant conditions. Sci. Hortic. 146, 52–58 (2012)

    Google Scholar 

  34. Richardson, A.E., Barea, J.M., McNeill, A.M., Prigent-Combaret, C.: Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321, 305–339 (2009)

    Google Scholar 

  35. Mendes, R., Kruijt, M., de Bruijn, I., Dekkers, E., van der Voort, M., Schneider, J.H., Piceno, Y.M., DeSantis, T.Z., Andersen, G.L., Bakker, P.A., Raaijmakers, J.M.: Deciphering the rhizosphere microbiome for disease-suppressive bacteria. Science 332, 1097–1100 (2011)

    Google Scholar 

  36. Kumari, P., Meena, M., Upadhyay, R.S.: Characterization of plant growth promoting rhizobacteria (PGPR) isolated from the rhizosphere of Vigna radiata (mung bean). Biocatal. Agric. Biotechnol. 16, 155–162 (2018)

    Google Scholar 

  37. Bushra, T., Anwar, K., Muhammad, T., Memoona, R., Muhammad, S.I.K., Naila, S., Khadija, A.: Bottlenecks in commercialisation and future prospects of PGPR. Appl. Soil Ecol. 121, 102–117 (2017)

    Google Scholar 

  38. Dong, X., Cai, M.: Handbook for Systematic Identification of Common Bacteria. China Science Press, Bei**g (2001)

    Google Scholar 

  39. Gupta, R., Ramnani, P.: Microbial keratinases and their prospective applications: an overview. Appl. Microbiol. Biotechnol. 70, 21–33 (2006)

    Google Scholar 

  40. Din, B.U., Sarfraz, S., **a, Y., Kamran, M.A., Javed, M.T., Sultan, T., Munis, M.F.H., Chaudhary, H.J.: Mechanistic elucidation of germination potential and growth of wheat inoculated with exopolysaccharide and ACC- deaminase producing Bacillus strains under induced salinity stress. Ecotoxicol. Environ. Saf. 183, 109466 (2019)

    Google Scholar 

  41. Sobucki, L., Ramos, R.F., Gubiani, E., Brunetto, G., Kaiser, D.R., Daroit, D.J.: Feather hydrolysate as a promising nitrogen-rich fertilizer for greenhouse lettuce cultivation. Int. J. Recycl. Org. Waste Agric. 8, 493–499 (2019). https://doi.org/10.1007/s40093-019-0281-7

    Article  Google Scholar 

  42. Joardar, J.C., Rahman, M.M.: Poultry feather waste management and effects on plant growth. Int. J. Recycl. Org. Waste Agric. 7, 183–188 (2018)

    Google Scholar 

  43. Gurav, R.G., Jadhav, J.P.: A novel source of biofertilizer from feather biomass for banana cultivation. Environ. Sci. Pollut. Res. 20, 4532–4539 (2013). https://doi.org/10.1007/s11356-012-1405-z

    Article  Google Scholar 

  44. Hadas, A., Kautsky, L.: Feather meal, a semi-slow release nitrogen fertilizer for organic farming. Fert. Res. 38, 165–170 (1994)

    Google Scholar 

  45. Gupta, R., Rajput, R., Sharma, R., Gupta, N.: Biotechnological applications and prospective market of microbial keratinases. Appl. Microbiol. Biotechnol. 97, 9931–9940 (2013)

    Google Scholar 

  46. Rai, S.K., Mukherjee, A.K.: Optimization for production of liquid nitrogen fertilizer from the degradation of chicken feather by iron-oxide (Fe3O4) magnetic nanoparticles coupled β-keratinase. Biocatal. Agric. Biotechnol. 4, 632–644 (2015)

    Google Scholar 

  47. Gianfreda, L., Antonietta, R.M., Piotrowska, A., Palumbo, G., Colombo, C.: Soil enzyme activities as affected by anthropogenic alterations: intensive agricultural practices and organic pollution. Sci. Total Environ. 341, 265–279 (2005)

    Google Scholar 

  48. Acosta-Martínez, V., Cruz, L., Sotomayor-Ramírez, D., Pérez-Alegría, L.: Enzyme activities as affected by soil properties and land use in a tropical watershed. Appl. Soil Ecol. 35, 35–45 (2007)

    Google Scholar 

  49. Wei, T., Zhang, P., Wang, K., Ding, R., Yang, B., Nie, J., Jia, Z., Han, Q.: Effects of wheat straw incorporation on the availability of soil nutrients and enzyme activities in semiarid areas. PLoS ONE 10, e0120994 (2015)

    Google Scholar 

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Acknowledgements

This research was sponsored by Shandong Modern Agricultural Industry Technology System (SDAIT-05-05), Shandong “Double Tops” Program (SYL2017XTTD15), Shandong Province Agricultural Major Application Technology Innovation Project (2018), Shandong Province Major Science and Technology Innovation Project (2017CXGC0311), and Agricultural Science and Technology Innovation Project funded by Shandong Academy of Agricultural Sciences.

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  1. College of Life Science, Shandong Agricultural University, Taian, 271018, Shandong, China

    Zhongtao Sun, Kexin Liu, **aoli Chi & Liying Liu

  2. College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, Shandong, China

    **anyao Li

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Sun, Z., Li, X., Liu, K. et al. Optimization for Production of a Plant Growth Promoting Agent from the Degradation of Chicken Feather Using Keratinase Producing Novel Isolate Bacillus pumilus JYL. Waste Biomass Valor 12, 1943–1954 (2021). https://doi.org/10.1007/s12649-020-01138-7

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