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Evaluation of GABA Production and Probiotic Activities of Enterococcus faecium BS5

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Abstract

Gamma-aminobutyric acid (GABA) is a principal inhibitory neurotransmitter in the central nervous system and is produced by irreversible decarboxylation of glutamate. It possesses several physiological functions such as neurotransmission, diuretic, and tranquilizer effects and also regulates cardiovascular functions such as blood pressure and heart rate in addition to playing a role in the reduction of pain and anxiety. The objective of this study was to evaluate the GABA producing ability and probiotic capability of certain lactic acid bacteria strains isolated from dairy products. Around sixty-four bacterial isolates were collected and screened for their ability to produce GABA from monosodium glutamate, among which nine isolates were able to produce GABA. The most efficient GABA producer was Enterococcus faecium BS5. Further, assessment of several important and desirable probiotic properties showed that Ent. faecium BS5 was resistant to acid stress, bile salt, and antibiotics. Ent. faecium BS5 may potentially be used for large-scale industrial production of GABA and also for functional fermented product development.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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The second author received financial assistance from DST-WOS A(SR/WOS-A/LS-458/2018).

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  1. Department of Microbial Biotechnology, Bharathiar University, Coimbatore, T.N., 641 046, India

    Sabna BS, Bency Thankappan, Ramasamy Mahendran, Gayathri Muthusamy & Jayaraman Angayarkanni

  2. Department of Biochemistry, Bharathiar University, Coimbatore, T.N., 641 046, India

    Daniel Raja Femil selta

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BS, S., Thankappan, B., Mahendran, R. et al. Evaluation of GABA Production and Probiotic Activities of Enterococcus faecium BS5. Probiotics & Antimicro. Prot. 13, 993–1004 (2021). https://doi.org/10.1007/s12602-021-09759-7

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