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Dietary Lactobacillus acidophilus and Mannan-Oligosaccharides Alter the Lipid Metabolism and Health Indices in Broiler Chickens

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Abstract

The effects of dietary Lactobacillus acidophilus (LBA) and mannan-oligosaccharide (MOS) supplementation on lipid metabolism and consequent lipid profile and health indices in broiler chicken were investigated in this study. Supplementation of 0.2% MOS along with either 106 or 107 LBA/g feed in broiler chicken downregulated hepatic expression of genes involved in lipogenesis, and upregulated expression of lipolytic genes. It caused decline of lipogenesis and increase of lipid oxidation which resulted in lower carcass fat content. None of the genes studied influenced fatty acid profile of chicken meat except the expression of stearoyl CoA (Δ9) desaturase-1 (SCD-1) whose upregulation increased monounsaturated fatty acid (MUFA) content at the cost of saturated fatty acid (SFA) content. The lipid metabolism indices of chicken meat such as ∆9 desaturase index (DI) increased in birds supplemented with 0.2% MOS along with either 106 or 107 CFU LBA/g feed, whereas no effect was observed on ∆5 + ∆6 DI. The supplementation of 0.2% MOS along with either 106 or 107 CFU LBA/g feed in birds improved the health indices of chicken meat due to upregulation of SCD-1 expression. The supplementation of 0.2% MOS along with either 106 or 107 CFU LBA/g feed in broiler chicken produced hypocholesterolemic and hypolipidemic effects with improved serum cardio-protective indices.

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Funding

This study was funded by the Department of Biotechnology, Ministry of Science and Technology, Government of India (Grant No. BT/PR9724/AAQ/1/571/2013).

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Authors and Affiliations

  1. ICAR-Central Avian Research Institute, Izatnagar, Bareilly, 243122, India

    Kapil Dev, Avishek Biswas & Nasir Akbar Mir

  2. Govind Ballabh Pant University of Agriculture & Technology, College of Veterinary & Animal Sciences, Pantnagar, 263145, India

    Jubeda Begum

  3. Sam Higginbottom University of Agriculture, Technology And Sciences, Allahabad, 211007, India

    Kapil Dev & Rajiv Kant

  4. CSIR-Indian Institute of Chemical Technology, Hyderabad, 500007, India

    Jyoti Kannoujia

  5. CSIR-Central Drug Research Institute, Lucknow, 226031, India

    T. Narender

  6. ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India

    Joyshikh Sonowal

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  1. Kapil Dev
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Correspondence to Avishek Biswas.

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All applicable institutional guidelines for the care and use of animals were followed. The experimental procedures carried out in this study were approved by the Institutional Animal Ethics Committee (IEAC) following the guidelines of “Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) 2012” established under the “Prevention of Cruelty of Animals Act 1960” of Indian Penal Code (18 September 2017/Project No. 11).

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Dev, K., Begum, J., Biswas, A. et al. Dietary Lactobacillus acidophilus and Mannan-Oligosaccharides Alter the Lipid Metabolism and Health Indices in Broiler Chickens. Probiotics & Antimicro. Prot. 13, 633–646 (2021). https://doi.org/10.1007/s12602-020-09717-9

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