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Inhibition of Salmonella enterica Cells in Deli-Type Salad by Enterocin AS-48 in Combination with Other Antimicrobials

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Abstract

The cyclic antibacterial peptide enterocin AS-48 acted synergistically with p-hydroxybenzoic acid methyl ester (PHBME) and with 2-nitropropanol against Salmonella enterica serovar Enteritidis CECT 4300 in Russian salad. In challenge tests on a cocktail of Salmonella strains (S. enterica ssp. enterica serotype Typhi CECT 409, S. enterica ssp. enterica serovar Choleraesuis CECT 915, S. enterica ssp. enterica serovar Enteritidis CECT 4300, S. enterica ssp. arizonae serovar Arizonae CECT 4395, and S. enterica ssp. salamae CECT 4000) in salad at 10°C, the combinations of PHBME and AS-48 (80 μg/g) or 2-nitropropanol and AS-48 (40 μg/g) reduced the concentrations of viable Salmonella from 4.27 to 4.75 log CFU/g down to the limit of detection for 7 days. Salmonella populations did not increase in control samples (without any antimicrobials or in the presence of AS-48 alone) probably due to the low pH of this type of salad and low temperature of incubation, but retained over 85% viability after 1 week. This work opens new possibilities to expand the spectrum of inhibition of enterocin AS-48 against Salmonella enterica.

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Acknowledgements

This work was supported by the Spanish Ministry of Education (research project AGL2005-07665-C02-02/ALI). We also acknowledge the Research Programme of the University of Jaén and the Research Plan of the Junta de Andalucía (research group AGR230).

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Authors and Affiliations

  1. Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus Las Lagunillas s/n, 23071, Jaén, Spain

    Antonio Cobo Molinos, Rosario Lucas López, Hikmate Abriouel, Nabil Ben Omar & Antonio Gálvez

  2. Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain

    Eva Valdivia

  3. Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain

    Eva Valdivia

Authors
  1. Antonio Cobo Molinos
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  2. Rosario Lucas López
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  3. Hikmate Abriouel
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  4. Nabil Ben Omar
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  5. Eva Valdivia
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  6. Antonio Gálvez
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Correspondence to Antonio Gálvez.

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Cobo Molinos, A., Lucas López, R., Abriouel, H. et al. Inhibition of Salmonella enterica Cells in Deli-Type Salad by Enterocin AS-48 in Combination with Other Antimicrobials. Probiotics & Antimicro. Prot. 1, 85–90 (2009). https://doi.org/10.1007/s12602-009-9005-z

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  • DOI: https://doi.org/10.1007/s12602-009-9005-z

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