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Pathogenicity and vegetative compatibility grou** among Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt

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Abstract

Thirty-nine isolates of Fusarium oxysporum f. sp. ciceri – the causal agent of chickpea (Cicer arietinum) wilt – collected from different parts of India and representing eight races of the pathogen, were analyzed for virulence and classified on the basis of vegetative compatibility grou** (VCG). The wilt incidence ranged from 24% to 100% on a highly susceptible cultivar JG 62. Six isolates, from Delhi, Gujarat, Karnataka, Punjab and Rajasthan and belonging to six different races of the pathogen, caused 100% wilt incidence. Five isolates belonging to four different races, namely, Foc 143 from Andhra Pradesh, Foc 161 from Chhattisgarh, Foc 146 from Karnataka, Foc 158 from Madhya Pradesh and Foc 50 from Rajasthan, caused low wilt incidence. For VCG analysis, nitrate non-utilizing mutants (nit) were obtained by culturing wild-type isolates on 2.5% potassium chlorate and selecting resistant sectors. Complementary nit mutants were paired in all possible combinations to determine varying degrees of heterokaryon formation within the isolates, which showed that most of the isolates were self-compatible. Pairing of all the mutants showed that the isolates included in the present study belonged to a single VCG (0280). Thus, in spite of variability in the virulence, the Indian populations of the pathogen have only one VCG.

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References

  • Alves-Santos, F. M., Benito, E. P., Eslava, A. P., & Diaz-Minguez, J. M. (1999). Genetic diversity of Fusarium oxysporum from common bean fields in Spain. Applied Environmental Microbiology, 65, 3335–3340.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Anagnostakis, S. L. (1982). Genetic analysis of Endothia parasitica: linkage data for four single genes and three vegetative compatibility types. Genetics, 102, 25–28.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Baayen, R. P., Forch, M. G., Waalwijk, C., Bonants, P. J. M., Loffler, H. J. M., & Roebroeck, E. (1998). Pathogenic, genetic and molecular characterization of Fusarium oxysporum f. sp. lilii. European Journal of Plant Pathology, 104, 887–894.

    Article  Google Scholar 

  • Bayraktar, H. H., Turkkan, M., & Dolar, F. S. (2010). Characterization of Fusarium oxysporum f. sp. cepae from onion in Turkey based on vegetative compatibility and rDNA RFLP analysis. Journal of Phytopathology, 158, 691–697.

    Article  CAS  Google Scholar 

  • Bosland, P. W., & Williams, P. H. (1987). An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility, and geographical origin. Canadian Journal of Botany, 65, 2067–2073.

    Article  Google Scholar 

  • Correll, J. C. (1991). The relationship between formae speciales, races, and vegetative compatibility groups in Fusarium oxysporum. Phytopathology, 81, 1061–1064.

    Google Scholar 

  • Correll, J. C., Klittich, C. J. R., & Leslie, J. F. (1987). Nitrate non-utilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology, 77, 1640–1646.

    Article  Google Scholar 

  • Correll, J. C., Puhalla, J. E., & Schneider, R. W. (1986). Vegetative compatibility groups among non-pathogenic root-colonizing strains of Fusarium oxysporum. Canadian Journal of Botany, 64, 2358–2361.

    Article  Google Scholar 

  • Dubey, S. C., Priyanka, K., Singh, V., & Singh, B. (2012). Race profiling and molecular diversity analysis of Fusarium oxysporum f. sp. ciceris causing wilt in chickpea. Journal of Phytopathology, 160, 576–587.

    Article  CAS  Google Scholar 

  • Dubey, S. C., & Singh, S. R. (2008). Virulence analysis and oligonucleotide fingerprinting to detect diversity among Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Mycopathologia, 165, 389–406.

    Article  CAS  PubMed  Google Scholar 

  • Dubey, S. C., Singh, S. R., & Singh, B. (2010). Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection, 45, 174–189.

    Article  Google Scholar 

  • Elmer, W. H., & Stephens, C. T. (1989). Classification of Fusarium oxysporum f. sp. asparagi into vegetative compatibility groups. Phytopathology, 79, 88–93.

    Article  Google Scholar 

  • Gilardi, G., Sendhilvel, V., Garibaldi, A., & Gullino, M. L. (2008). Lamb’s lettuce (Valerianella olitoria): new host of Fusarium oxysporum f. sp. conglutinans. Journal of Plant Diseases and Protection, 115, 229–233.

    Google Scholar 

  • Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research (pp. 139–153). Singapore: John Wiley & Sons.

    Google Scholar 

  • Haware, M. P., & Nene, Y. L. (1982a). Symptomless carriers of the chickpea wilt Fusarium. Plant Disease, 66, 250–251.

    Article  Google Scholar 

  • Haware, M. P., & Nene, Y. L. (1982b). Races of Fusarium oxysporum f. sp. ciceri. Plant Disease, 66, 809–810.

    Article  Google Scholar 

  • Jacobson, D. J., & Gordon, T. R. (1988). Vegetative compatibility and self-incompatibility within Fusarium oxysporum f. sp. melonis. Phytopathology, 78, 668–672.

    Article  Google Scholar 

  • Jimenez-Diaz, R. M., Alcala-Jimenez, A. R., Hervar, A., & Trapero-Casas, J. L. (1993). Pathogenic variability and host resistance in the Fusarium oxysporum f. sp. ciceri / Cicer arietinum pathosystem. pp. 87-94. In E. Arseniuk & T. Goral (Eds.), Proceedings of Third European Seminar: Fusarium – mycotoxins taxonomy, pathogenicity and host resistance. Rodzikov: Plant Breeding and Acclimatization Institute.

    Google Scholar 

  • Jiménez-Gasco, M. M., Milgroom, M. G., & Jiménez-Díaz, R. M. (2002). Gene genealogies support Fusarium oxysporum f. sp. ciceris as a monophyletic group. Plant Pathology, 51, 72–77.

    Article  Google Scholar 

  • Katan, T. (1999). Current status of vegetative compatibility groups in Fusarium oxysporum. Phytoparasitica, 27, 51–64.

    Article  Google Scholar 

  • Katan, T., & Katan, J. (1988). Vegetative compatibility grou** of Fusarium oxysporum f. sp. vasinfectum from tissue and the rhizosphere of cotton plants. Phytopathology, 78, 852–855.

    Article  Google Scholar 

  • Katan, T., & Katan, J. (1999). Vegetative compatibility grou** in Fusarium oxysporum f.sp. radicis-lycopersici from the UK, the Netherlands, Belgium and France. Plant Pathology, 48, 541–549.

    Article  Google Scholar 

  • Katan, T., Zamir, D., Sarfatti, M., & Katan, J. (1991). Vegetative compatibility groups and subgroups in Fusarium oxysporum f.sp. radicis-lycopersici. Phytopathology, 81, 255–262.

    Article  CAS  Google Scholar 

  • Kiprop, E. K., Mwangombe, A. W., Baudoin, J. P., Kimani, P. M., & Mergeai, G. (2002). Cultural characteristics, pathogenicity and vegetative compatibility of Fusarium udum isolates from pigeonpea (Cajanus cajan (L.) Millsp.) in Kenya. European Journal of Plant Pathology, 108, 147–154.

    Article  Google Scholar 

  • Larkin, R. P., Hopkins, D. L., & Martin, F. N. (1990). Vegetative compatibility within Fusarium oxysporum f. sp. niveum and its relationship to virulence, aggressiveness and race. Canadian Journal of Microbiology, 36, 352–358.

    Article  Google Scholar 

  • Mohammadi, A., Nejad, R. F., & Mofrad, N. N. (2012). Fusarium verticillioides from sugarcane, vegetative compatibility groups and pathogenicity. Plant Protection Science, 48, 80–84.

    CAS  Google Scholar 

  • Nogales-Moncada, A. M., Jimenez Dıaz, R. M., & Perez Artes, E. (2009). Vegetative compatibility groups in Fusarium oxysporum f. sp. ciceris and F. oxysporum non-pathogenic to chickpea. Journal of Phytopathology, 157, 729–735.

    Article  Google Scholar 

  • Pasquali, M., Marena, L., Gullino, M. L., & Garibaldi, A. (2004). Vegetative compatibility grou** of Fusarium wilt pathogen of paris daisy (Argyranthemum frutescens L.). Journal of Phytopathology, 152, 257–259.

    Article  Google Scholar 

  • Ploetz, R. C., & Shepard, E. S. (1989). Fusarial wilt of banana in Florida. Mycological Research, 93, 242–245.

    Article  Google Scholar 

  • Puhalla, J. E. (1985). Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Canadian Journal of Botany, 62, 540–545.

    Article  Google Scholar 

  • Roebroeck, E. J. A., & Mes, J. J. (1992). Physiological races and vegetative compatibility groups within Fusarium oxysporum f. sp. gladioli. Netherlands. Journal of Plant Pathology, 98, 57–64.

    Google Scholar 

  • Zamani, M. R., Motallebi, M., & Rostamian, A. (2004). Characterization of Iranian isolates of Fusarium oxysporum on the basis of RAPD analysis, virulence and vegetative compatibility. Journal of Phytopathology, 152, 449–453.

    Article  CAS  Google Scholar 

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Acknowledgments

The study was supported by ICAR and the Indian Agricultural Research Institute, New Delhi, India.

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Authors and Affiliations

  1. Division of Crop Protection, Indian Institute of Pulses Research, Kanpur, 208024, India

    Parasappa R. Saabale

  2. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Sunil C. Dubey

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  1. Parasappa R. Saabale
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  2. Sunil C. Dubey
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Correspondence to Parasappa R. Saabale.

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Saabale, P.R., Dubey, S.C. Pathogenicity and vegetative compatibility grou** among Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Phytoparasitica 42, 465–473 (2014). https://doi.org/10.1007/s12600-014-0383-8

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