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Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-stain-positive, halophilic, rod-shaped, non-motile, spore forming bacterium, strain NKC1-2T, was isolated from kimchi, a Korean fermented food. Comparative analysis based on 16S rRNA gene sequence demonstrated that the isolated strain was a species of the genus Virgibacillus. Strain NKC1-2T exhibited high level of 16S rRNA gene sequence similarity with the type strains of Virgibacillus xinjiangensis SL6-1T (96.9%), V. sediminis YIM kkny3T (96.8%), and V. salarius SA-Vb1T (96.7%). The isolate grew at pH 6.5–10.0 (optimum, pH 8.5–9.0), 0.0–25.0% (w/v) NaCl (optimum, 10–15% NaCl), and 15–50°C (optimum, 37°C). The major menaquinone in the strain was menaquinone-7, and the main peptidoglycan of the strain was meso-diaminopimelic acid. The predominant fatty acids of the strain were iso-C14:0, anteisio-C15:0, iso- C15:0, and iso-C16:0 (other components were < 10.0%). The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G + C content of NKC1-2T was 42.5 mol%. On the basis of these findings, strain NKC1-2T is proposed as a novel species in the genus Virgibacillus, for which the name Virgibacillus kimchii sp. nov. is proposed (=KACC 19404T =JCM 32284T). The type strain of Virgibacillus kimchii is NKC1-2T.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea

    Young Joon Oh, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, Jieun Lee, NamHee Kim, Mi-Young Shin, Hyo Kyeong Park & Hak-Jong Choi

  2. Division of Animal Science, Chonnam National University, Gwangju, 61186, Republic of Korea

    Mi-Young Shin

  3. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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  1. Young Joon Oh
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  2. Ja-Young Jang
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  3. Seul Ki Lim
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  5. Jieun Lee
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Correspondence to Hak-Jong Choi.

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Oh, Y.J., Jang, JY., Lim, S.K. et al. Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi. J Microbiol. 55, 933–938 (2017). https://doi.org/10.1007/s12275-017-7386-3

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  • DOI: https://doi.org/10.1007/s12275-017-7386-3

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