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Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Map** Method

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Abstract

Abnormal postoperative neurobehavioral performance (APNP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as their potential association with APNP are still not well understood. Here, we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The abnormal neurobehavioral performance occurred at 6 h and/or 9 h, and recovered at 24 h after anesthesia/surgery. Compared with the control group, the altered metabolite of the model group at 6 h was aspartate (Asp), and the difference was mainly displayed in the cortex; while significant changes at 9 h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu). All changes returned to baseline at 24 h. The altered metabolic changes could have occurred as a result of the acute APNP, and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.

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  • 04 April 2020

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Acknowledgements

We would like to express our gratitude to Mrs. **** An (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences) for her help in housing the animals. This work was supported by grants from the National Natural Science Foundation of China (8187051484, 8157050329, and 81600933), the Interdisciplinary Medicine Seed Fund of Peking University (BMU2017MC006), and the Youth Innovation Promotion Association of the Chinese Academy of Sciences, China (Y6Y0021004).

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Authors and Affiliations

  1. Department of Anesthesiology, Peking University Third Hospital, Bei**g, 100191, China

    Taotao Liu, Zhengqian Li, **dan He, Ning Yang, Dengyang Han, Yue Li & **angyang Guo

  2. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China

    Taotao Liu, Huili Liu, Fuqiang Xu & Jie Wang

  3. Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xuebi Tian & Hongbing **ang

  4. School of Human and Social Sciences, University of West London, Middlesex, TW89GA, UK

    Anne Manyande

  5. University of Chinese Academy of Sciences, Bei**g, 100049, China

    Fuqiang Xu & Jie Wang

  6. The Second Hospital of Shijiazhuang, Shijiazhuang, 050051, China

    Jie Wang

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  1. Taotao Liu
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Liu, T., Li, Z., He, J. et al. Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Map** Method. Neurosci. Bull. 36, 25–38 (2020). https://doi.org/10.1007/s12264-019-00414-4

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