Abstract
Bovine diarrhea is a major concern in the global bovine industry because it can cause significant financial damage. Of the many potential infectious agents that can lead to bovine diarrhea, bovine rotavirus (BRV) is a particular problem due to its high transmissibility and infectivity. Therefore, it is important to prevent the proliferation of BRV using an early detection system. This study developed an affinity peptide-based electrochemical method for use as a rapid detection system for BRV. A BRV-specific peptide was identified via the phage display technique and chemically synthesized. The synthetic peptide was immobilized on a gold electrode through thiol-gold interactions. The performance of the BRV specific binding peptides was evaluated using square wave voltammetry. The developed detection system exhibited a low detection limit (5 copies/mL) and limit of quantitation (2.14 × 102 copies/mL), indicating that it is a promising sensor platform for the monitoring of BRV.
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Acknowledgements
This research was supported by the Chung-Ang University Graduate Research Scholarship, 2021. This study was also supported by the Animal Disease Management Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (120090-2) (T.J. Park).
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Cho, C.H., Park, T.J. & Park, J.P. Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus. Biotechnol Bioproc E 27, 607–614 (2022). https://doi.org/10.1007/s12257-022-0044-6
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DOI: https://doi.org/10.1007/s12257-022-0044-6