Log in

Bioinformatic analysis of BBTV satellite DNA in Hainan

  • Published:
Virologica Sinica

Abstract

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiation protein of BBTV DNA1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bell K E, Dale J L, Ha C V, et al. 2002. Characterisation of Rep-encoding compenents associated with banana bunchy top nanovirus in Vietnam. Arch Virol, 147:695–707.

    Article  PubMed  CAS  Google Scholar 

  2. Burns T M, Harding R M, Dale J L. 1995. The genome organization of Banana bunchy top virus: analysis of six ssDNA components. J Gen Virol, 76: 1471–1482.

    Article  PubMed  CAS  Google Scholar 

  3. Dale J L. 1987. Banana bunchy top: aneconomically important tropical plant virus disease. Adv Virus Res, 33: 301–325.

    Article  PubMed  CAS  Google Scholar 

  4. Fauquet C M, Mayo M A, Maniloff J, et al. 2005. Virus Taxonomy, Classification and Nomenclature of Viruses. In: Eighth Report of the International Committee on the Taxonomy of Viruses. Amsterdam, Boston, Heidelierg: Elsevier Academic Press.

    Google Scholar 

  5. Harding R M, Burns T M, Dale J L. 1991. Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. J Gen Virol, 72:225–230.

    Article  PubMed  CAS  Google Scholar 

  6. Harding R M, Burns T M, Hafner G J, et al. 1993. Nucleotide-sequence of one-component of the Banana bunchy top virus genome contains a putative replicase gene. J Gen Virol, 74: 323–328.

    Article  PubMed  CAS  Google Scholar 

  7. Hermann S R, Becker D K, Harding R M, et al. 2001. Promoters derived from banana bunchy top virus-associated components S1 and S2 drive transgene expression in both tobacco and banana. Plant Cell Rep, 20: 642–646.

    Article  CAS  Google Scholar 

  8. Horser C L, Karan M, Harding R M, et al. 2001. Additional Rep-encoding DNAs associated with banana bunchy top virus. Arch Virol, 146: 71–86.

    Article  PubMed  CAS  Google Scholar 

  9. Karan M, Harding R M, Dale J L. 1997. Association of banana bunchy top virus DNA components 2 to 6 with banana bunchy disease. Mol Plant Pathol, On-line: 1–16.

  10. Wu R Y, You L R, Soong T S. 1994. Nucleotide sequence of two circular single-stranded DNA’s associated with banana bunchy top virus. Phytopathology, 84: 952–958.

    Article  CAS  Google Scholar 

  11. Yu N T, Zhang Y L, Wang J H, et al. 2011. Characteristics analysis of banana bunchy top virus Rep protein and establishment of its purification system. J Agric Sci Technol, 13(2): 31–37.

    CAS  Google Scholar 

  12. Zhou Z Z, Lin Q Y, **e L H, et al. 1993. Studies on banana bunchy top I. Incidence, epidemics and distribution of the disease in Fujian. J Fujian Agric Univ (Nat Sci ed), 22(3): 305–310.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhi-xin Liu.

Additional information

Foundation item: The Central Level, Scientific Research Institutes for Basic R & D Special Fund Business (ITBB110303).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, Nt., Feng, Tc., Zhang, Yl. et al. Bioinformatic analysis of BBTV satellite DNA in Hainan. Virol. Sin. 26, 279–284 (2011). https://doi.org/10.1007/s12250-011-3196-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12250-011-3196-7

Key words

Navigation