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Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

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Abstract

Globodera rostochiensis and Globodera pallida are parasitic root cyst nematodes of potato (PCN), which significantly reduce yield and quality. The genetics and available molecular markers should make resistance to nematodes an excellent candidate for marker assisted selection. The study presents results of testing the suitability of known molecular markers for detection of resistance in a set of cultivars. We revealed some inconsistencies in genealogical data of the cultivars and showed inconsistent usefulness in detecting resistant cultivars. The marker TG689 was identified in almost all cultivars resistant to G. rostochiensis and together with other markers was used for verifying the resistance in a group of breeding lines. The marker TG689 was the most effective. However, the efficiency of tested markers in selecting resistant individuals might be inadequate. In the case of quantitative resistance to G. pallida, marker SPUD1636 was found in resistant cultivars, and not in susceptible cultivars, but turned out to be inefficient in selecting resistant breeding lines.

Resumen

Globodera rostochiensis y Globodera pallida son nematodos de quiste parásitos de la raíz de la papa (PCN) que reducen significativamente el rendimiento y la calidad. La genética y los marcadores moleculares disponibles, deberían de hacer de la resistencia a nematodos un excelente candidato para selección asistida con marcadores. El estudio presenta resultados de probar lo deseable de los marcadores moleculares conocidos para la detección de resistencia en un grupo de variedades. Revelamos algunas inconsistencias en datos genealógicos de las variedades y mostramos uso inconsistente en la detección de variedades resistentes. El marcador TG689 se identificó en casi todas las variedades resistentes a G. rostochiensis y junto con otros marcadores se usó para verificar la resistencia en un grupo de líneas de mejoramiento. El marcador TG689 fue el más efectivo. No obstante, podría ser inadecuada la eficiencia de los marcadores probados en la selección de individuos resistentes. En el caso de resistencia cuantitativa a G. pallida, se encontró el marcador SPUD1636 en variedades resistentes y no en los susceptibles, pero resultó ser ineficiente en la selección de líneas de mejoramiento resistentes.

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Acknowledgements

This work was partly supported by the project 4-3-00-7-02 of the Ministry of Agriculture and Rural Development.

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Authors and Affiliations

  1. Plant Breeding and Acclimatization Institute, Research Centre Młochów, Platanowa 19, 05-831, Młochów, Poland

    Dorota Milczarek & Bogdan Flis

  2. Plant Breeding and Acclimatization Institute, Research Centre Radzików, 05-870, Błonie, Poland

    Anna Przetakiewicz

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  1. Dorota Milczarek
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Correspondence to Dorota Milczarek.

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Milczarek, D., Flis, B. & Przetakiewicz, A. Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp. Am. J. Pot Res 88, 245–255 (2011). https://doi.org/10.1007/s12230-011-9189-0

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