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Fabricating a Novel Raman Spectroscopy-Based Aptasensor for Rapidly Sensing Salmonella typhimurium

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Abstract

A novel surface-enhanced Raman scattering (SERS) response emerging from aptamers, complementary DNA (cDNA), p-aminothiophenol (PATP), and Au nanorods (GNRs) for detection of foodborne pathogens sensitivity is proposed here. In the presence of Salmonella typhimurium (ST) and cDNA simultaneously, ST and cDNA would competitively combine with the S. typhimurium aptamer (STA), inducing a highly conformation changes of STA. Accordingly, STA no longer stabilizes the GNRs in salt solution, leading to the varying aggregation extent of GNRs. The varying GNR aggregation will give rise to the plasmonic coupling and display a strong SERS signal, which can be distinctly reflected by the attached PATP via a transition of signals typical from ST peaks to PATP peaks. Under optimal conditions, the SERS intensity was observed to increase linearly with ST concentration from 56 to 56 × 107 cfu/mL (R 2 = 0.971), with a LOD of 9 cfu/mL. Additionally, this aptasensor exhibits a high selectivity to other similar pathogens, and the ability of the bioassay to detect ST was also confirmed in adulterated milk samples.

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Acknowledgements

This work has been financially supported by the National Natural Science Foundation of China (31471646), the Key R&D Program of Jiangsu Province (BE2015302), Postgraduate Innovative Program for Higher Education Institutions in Jiangsu Province (KYLX16_0913), the Natural Science Foundation of Jiangsu Province (Youth) (BK20150502), the China Postdoctoral Science Foundation (2015 M571698), and the Advanced Talents Science Foundation of Jiangsu University (15JDG064).

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  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Huanhuan Li, Quansheng Chen, Qin Ouyang & Jiewen Zhao

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  1. Huanhuan Li
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  2. Quansheng Chen
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  3. Qin Ouyang
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Correspondence to Quansheng Chen.

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Huanhuan Li declares that she has no conflict of interest. Quansheng Chen declares that he has no conflict of interest. Qin Ouyang declares that she has no conflict of interest. Jiewen Zhao declares that he has no conflict of interest.

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Li, H., Chen, Q., Ouyang, Q. et al. Fabricating a Novel Raman Spectroscopy-Based Aptasensor for Rapidly Sensing Salmonella typhimurium . Food Anal. Methods 10, 3032–3041 (2017). https://doi.org/10.1007/s12161-017-0864-8

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