Abstract
A novel method was studied for determination of 20 free amino acids in asparagus tin by high-performance liquid chromatography with pre-column derivatization. Derivatization of the samples was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF), and the SPE cartridge was used for purification and enrichment of the analytes. The derivatization conditions and the influence of elution composition on the separation were investigated. The reaction of amino acids with CNBF was completed in pH 9.0 borate buffer. The separation of amino acids was achieved at room temperature within 60 min by gradient elution mode with triethylamine in mobile phase, and the flow rate is 0.32 mL min−1 constantly. The method correlation coefficient was from 0.9975 to 1.0000 in concentrations ranging from 20 to 2000 μmol L−1, except asparagine (from 100 to 10000 μmol L−1). The detection limits of amino acids were from 1.2 to 6.0 μmol L−1, with a signal-to-noise ratio of three times. The calculated recoveries of the proposed method were from 81.4% to 109.4%, and relative standard deviations were 0.48–3.94% in application to the quantitative determination of free amino acids in asparagus tin samples. The present method is reliable and sensitive that allows fast analysis of free amino acids in asparagus tin, which makes it suitable for further study of free amino acids in other asparagus foods.
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References
Blau K, King G (1977) Handbook of derivatives for chromatography. Heyden, London
Bosch L, Alegría A, Farré R (2006) Application of the 6-aminoquinolyl-N-hydroxysccinimidyl carbamate (AQC) reagent to the RP-HPLC determination of amino acids in infant foods. J Chromatogr B 831:176–183
Dawson LA, Organ AJ, Winter P, Lacroix LP (2004) Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns. J Chromatogr B 807:235–241
Einarsson S, Josefsson B, Lagerkvist S (1983) Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. J Chromatogr Atogr 282:609–618
Fan XJ, You JM, Kang JW, Ou QY, Zhu QC (1998) New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization. Anal Chim Acta 367:81–91
Fernández-Fígares I, Rodríguez LC, González-Casado A (2004) Effect of different matrices on physiological amino acids analysis by liquid chromatography: evaluation and correction of the matrix effect. J Chromatogr B 799:73–79
Fiedler HP, Plaga A (1987) Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization. J Chromatogr Atogr 386:229–241
Gatti R, Gioia MG, Di Pietra AM, Cavrini V (2001) Analysis of phenols in pharmaceuticals by liquid chromatography after pre-column labelling and on-line post-column photochemical derivatization. Anal Chim Acta 447:89–99
Gioia MG, Andreatta P, Boschetti S, Gatti R (2007) Development and validation of a liquid chromatographic method for the determination of branched-chain amino acids in new dosage forms. J Pharm Biomed Anal 45:456–464
Knapp DR (1979) Handbook of analytical derivatization reactions. Wiley, New York
Kőrös Á, Varga Z, Molnár-Perl I (2008) Simultaneous analysis of amino acids and amines as their o-phthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography. J Chromatogr A 1203:146–152
Li M, Yang ZX, **e B, Shan LJ, Dong JJ (2007) Determination 21 free amino acids in beer by HPLC with AQC precolumn derivatization. Chin J Chromatogr 25:939–941
Lozanov V, Benkova B, Mateva L, Petrov S, Popov E, Slavov C, Mitev V (2007) Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile. J Chromatogr B 860:92–97
López-Cervantes J, Sánchez-Machado DI, Rosas-Rodríguez JA (2006) Analysis of free amino acids in fermented shrimp waste by high-performance liquid chromatography. J Chromatogr A 1105:106–110
Naval MV, Gómez-Serranillos MP, Carretero ME, De Arce C (2006) Value of high-performance liquid chromatographic analysis of amino acids in the determination of Panax ginseng radix extract effect in cultured neurons. J Chromatogr A 1121:242–247
Pereira V, Pontes M, Câmara JS, Marques JC (2008) Simultaneous analysis of free amino acids and biogenic amines in honey and wine samples using in loop orthophthalaldeyde derivatization procedure. J Chromatogr A 1189:435–443
Qian K, Tang T, Shi TY, Wang F, Li JQ, Cao YS (2009) Residue determination of glyphosate in environmental water samples with high-performance liquid chromatography and UV detection after derivatization with 4-Chloro-3, 5-Dinitrobenzotrifluoride. Anal Chim Acta 635:222–226
Shi TY, Tang T, Qian K, Wang F, Li JQ, Cao YS (2009) High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3, 5-dinitrobenzo- trifluoride. Anal Chim Acta 654:154–161
Sun Y, Liu PF, Wang D, Li JQ, Cao YS (2009) Determination of amitrole in environmental water samples with precolumn derivatization by high-performance liquid chromatography. J Agr Food Chem 57:4540–4544
Tang T, Shi TY, Qian K, Li PL, Li JQ, Cao YS (2009) Determination of biogenic amines with pre-chromatographic derivatization by high performance liquid chromatography. J Chromatogr B 877:507–512
Todoroki K, Etoh H, Yoshida H, Nohta H, Yamaguchi M (2009) A fluorous tag-bound fluorescence derivatization reagent, F-trap pyrene, for reagent peak-free HPLC analysis of aliphatic amines. Anal Bioanal Chem 394:321–327
Wan H, Andersson PE, Èm AE, Blomberg LG (1995) Direct and indirect chiral separation of amino acids by capillary electrophoresis. J Chromatogr A 704:179
Yang CL, Jiang XM, Guo LY, Zhang HX, Liu MC (2007) Analysis of free amino acids in islets of Langerhans by high-performance liquid chromatography using pre-column derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole. J Sep Sci 30:3154–3163
Zacharis CK, Theodoridis GA, Voulgaropoulos AN (2006) Coupling of sequential injection with liquid chromatography for the automated derivatization and on-line determination of amino acids. Talanta 69:841–847
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This work was supported by a scientific project of the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (No. 2010IK157).
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Li, W., Hou, M., Cao, Y. et al. Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization. Food Anal. Methods 5, 62–68 (2012). https://doi.org/10.1007/s12161-011-9197-1
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DOI: https://doi.org/10.1007/s12161-011-9197-1