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Short Ozonation of Lignocellulosic Waste as Energetically Favorable Pretreatment

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Abstract

Lignocellulosic waste (here municipal trimmings) is a promising sustainable feedstock for ethanol production, but requires costly and polluting pretreatment, often resulting in toxic by-products. Ozonation, nonpolluting, effective pretreatment method, is not used commercially due to high energy requirements of ozone production at high ozone doses needed. Our results, however, demonstrated that low-dose ozonation (15 min, accumulated TOD = 318 mg L−1) of water-submerged waste resulted in improved enzymatic saccharification efficiency (31% of cellulose) compared to a non-ozonated sample (12%) although only 20% of the lignin was removed. Ozonation up to 90 min resulted in better conversion however exceptionally long ozonation (6 h and beyond) resulted in reduced conversion. These results suggest that contrary to common hypothesis, short ozonation could offer an effective and feasible pretreatment method for high sugar release without the need for delignification. In addition, the ozonation process was accompanied by changes in absorbance, mainly at 280 nm, making it a useful tool for process monitoring. Net calculated energy balance was positive for all ozonation regimes, with increased process efficiency at lower ozone doses. Furthermore, ozonation can be generated on-site and on demand, enabling decentralized pretreatment operated near the feed source, thus overcoming transportation costs.

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Acknowledgements

We would like to thank Dr. Alex Golberg, Tel Aviv University, for his enormous help in the HPIC data analysis and for his advice. We also wish to thank Du-Pont Company for their donation of enzymes.

Funding

This research was conducted in the framework of the Israeli Ministry of National Infrastructure, Energy and Water Resources grant number 214-11-006, the Israeli Ministry of Environmental Protection grant number 132-3-4, and a scholarship from the Israeli Ministry of Science and Technology.

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Authors and Affiliations

  1. Porter School of Environmental Studies (PSES), Faculty of Exact Sciences, Tel Aviv University, 69978, Tel Aviv, Israel

    Yan Rosen

  2. School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, 69978, Tel Aviv, Israel

    Hadas Mamane

  3. Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa – Oranim, 3600600, Tivon, Israel

    Yoram Gerchman

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  1. Yan Rosen
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Correspondence to Yoram Gerchman.

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Rosen, Y., Mamane, H. & Gerchman, Y. Short Ozonation of Lignocellulosic Waste as Energetically Favorable Pretreatment. Bioenerg. Res. 12, 292–301 (2019). https://doi.org/10.1007/s12155-019-9962-3

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