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Stem Cells from Human Exfoliated Deciduous Teeth Modulate Early Astrocyte Response after Spinal Cord Contusion

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A Correction to this article was published on 16 June 2018

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Abstract

The transplantation of stem cells from human exfoliated deciduous teeth (SHED) has been studied as a possible treatment strategy for spinal cord injuries (SCIs) due to its potential for promoting tissue protection and functional recovery. The aim of the present study was to investigate the effects of the early transplantation of SHED on glial scar formation and astrocytic reaction after an experimental model of SCI. Wistar rats were spinalized using the NYU Impactor. Animals were randomly distributed into three groups: control (naive) (animal with no manipulation); SCI (receiving laminectomy followed by SCI and treated with vehicle), and SHED (SCI rat treated with intraspinal SHED transplantation, 1 h after SCI). In vitro investigation demonstrated that SHED were able to express mesenchymal stem cells, vimentin and S100B markers, related with neural progenitor and glial cells, respectively. The acute SHED transplantation promoted functional recovery, measured as from the first week after spinal cord contusion by Basso, Beattie, and Bresnahan scale. Twenty-four and 48 h after lesion, flow cytometry revealed a spinal cord vimentin+ cells increment in the SHED group. The increase of vimentin+ cells was confirmed by immunofluorescence. Moreover, the bioavailability of astrocytic proteins such as S100B and Kir4.1 shown to be increased in the spinal cord of SHED group, whereas there was a glial scar reduction, as indicated by ELISA and Western blot techniques. The presented results support that SHED act as a neuroprotector agent after transplantation, probably through paracrine signaling to reduce glial scar formation, inducing tissue plasticity and functional recovery.

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  • 16 June 2018

    The authors hereby declare that the Figure 4 in page eight of the paper “Stem cells from human exfoliated deciduous teeth modulate early astrocyte response after spinal cord contusion” authored by Fabrício Nicola and colleagues (DOI: 10.1007/s12035-018-1127-4) was mistakenly included.

Abbreviations

APC:

Allophycocyanin

AQP4:

Aquaporin 4

BBB:

Basso, Beattie, and Bresnahan scale

FITC:

Fluorescein isothiocyanate

GFAP:

Glial fibrillary acidic protein

Kir:

Inward rectifying potassium channel

MASCIS:

Multicenter Animal Spinal Cord Injury Study

MSCs:

Mesenchymal stem cells

PE:

Phycoerythrin

PMSF:

Phenylmethyl-sulphonyl fluoride

SCI:

Spinal cord injury

SHED:

Stem cells from human exfoliated deciduous teeth

S100B:

Calcium-binding protein

TBS:

Tris-buffered saline

Tx:

Triton X-100

Vimentin:

Progenitor neural cells

βIII-tubulin:

Neuronal microtubule protein

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Acknowledgements

This work was supported by funds from the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico do Brasil (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Stem Cell Research Institute.

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Authors and Affiliations

  1. Post Graduate Program in Neuroscience, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Fabrício Nicola, Marília Rossato Marques & Felipe Odorcyk

  2. Department of Biochemistry, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul, R. Ramiro Barcelos, 2600 anexo, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil

    Fabrício Nicola, Marília Rossato Marques, Felipe Odorcyk, Letícia Petenuzzo, Dirceu Aristimunha, Adriana Vizuete, Eduardo Farias Sanches, Carlos-Alberto Gonçalves & Carlos Alexandre Netto

  3. Hematology and Stem Cell Laboratory, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Daniela Pavulack Pereira, Natasha Maurmann & Patricia Pranke

  4. Post Graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Natasha Maurmann & Patricia Pranke

  5. Stem Cell Research Institute, Porto Alegre, Brazil

    Patricia Pranke

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Correspondence to Fabrício Nicola.

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All procedures were in accordance with the Guide for the Care and Use of Laboratory Animals adopted by the National Institute of Health (USA) and with the Federation of Brazilian Societies for Experimental Biology and with the Brazilian Law for Laboratory Animals care no 11.794. The experimental study was approved by the Research Ethics Committee of the University (#26116). The procedures for obtaining and isolate the SHED were approved by the Ethics Committee of the Universidade Federal do Rio Grande do Sul (#296/08).

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Nicola, F., Marques, M.R., Odorcyk, F. et al. Stem Cells from Human Exfoliated Deciduous Teeth Modulate Early Astrocyte Response after Spinal Cord Contusion. Mol Neurobiol 56, 748–760 (2019). https://doi.org/10.1007/s12035-018-1127-4

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