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Increased Expression of Small Heat Shock Protein αB-crystallin After Intracerebral Hemorrhage in Adult Rats

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Abstract

αB-crystallin (αBC) is involved in diverse cellular activities. Previous studies demonstrated that αBC had anti-apoptotic and proliferation-promoting effects in multiple diseases. Here, we explored the αBC’s roles in the pathophysiology of intracerebral hemorrhage (ICH). An ICH rat model was established and assessed by behavioral tests. Using Western blot and immunohistochemistry, significant up-regulation of αBC was found in neurons and astrocytes in brain areas surrounding the hematoma following ICH. Increase of αBC expression was found to be accompanied by the increased expression of proliferating cell nuclear antigen (PCNA), p53, Bax, and active-caspase-3. αBC was co-localized with PCNA in astrocytes or active-caspase-3 in neurons, suggesting its role in astrocyte proliferation and neuronal apoptosis. Our in vitro study, using αBC RNA interference in PC12 cells, indicated that αBC might exert its anti-apoptotic function in neuronal apoptosis. Thus, αBC may play a role in protecting the brain from secondary damage following ICH.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant no. 21077061) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People’s Republic of China

    Kaifu Ke, Lei Li, Ying Rui, Heyi Zheng, **ang Tan, Wei Xu, Gang Cui, Guangfei Xu & Maohong Cao

  2. Department of Psychiatry, Affiliated Mental and Health Center of Nantong University, Nantong, Jiangsu Province, People’s Republic of China

    Jianhua Cao & Jian Xu

Authors
  1. Kaifu Ke
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  2. Lei Li
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  3. Ying Rui
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  4. Heyi Zheng
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  5. **ang Tan
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  6. Wei Xu
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  7. Jianhua Cao
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  8. Jian Xu
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  9. Gang Cui
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  10. Guangfei Xu
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  11. Maohong Cao
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Correspondence to Guangfei Xu or Maohong Cao.

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Kaifu Ke and Lei Li contributed equally to this work.

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Ke, K., Li, L., Rui, Y. et al. Increased Expression of Small Heat Shock Protein αB-crystallin After Intracerebral Hemorrhage in Adult Rats. J Mol Neurosci 51, 159–169 (2013). https://doi.org/10.1007/s12031-013-9970-2

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  • DOI: https://doi.org/10.1007/s12031-013-9970-2

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